Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184705_xu9k TTACAAAATCTAAAAGAAGTAACACATTCTGG(+AGGACA)GTACCATCGAGGCATTTAGATTGCTCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240613184703_1i1j GTCCTGCCTACCAACTAGGGCCAAGTCTGCTG(+AGGACA)GATCCTGCCAGTGGGGTCTTAAGTATAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613184702_8lln CGGGATCTTTATACTTAAGACCCCACTGGCAG(+AGGACA)GATCCAGCAGACTTGGCCCTAGTTGGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240613184700_qa5s AAATTGTTCTGTGTATTTGTCACATATTTAAG(+AGGACA)GATCCACGCTTCTTATATTTTCTTTTATTAT NGG 10 8 17 8 24 0 100 5 10
    240613184659_rred AACTTATAATAAAAGAAAATATAAGAAGCGTG(+AGGACA)GATCCTTAAATATGTGACAAATACACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240613184657_0r5b GATGTTCATTATTCCTAAATAGCAGCTCAAAG(+AGGACA)GATCCCTTTGCTACACAAGCGCAATGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240613184656_g9k7 CTTAGGTTTTCATTGCGCTTGTGTAGCAAAGG(+AGGACA)GATCCTTTGAGCTGCTATTTAGGAATAATGA NGG 10 8 17 8 24 0 100 5 10
    240613184655_4769 AATGTGTGTATTTCTTTATCTCTTACTCTATG(+AGGACA)GTACCATGAGATCATTCAGCCGTCCTTGTCA NGG 10 8 17 8 24 0 100 5 10
    240613184653_3oaf ACCTTTGACAAGGACGGCTGAATGATCTCATG(+AGGACA)GTACCATAGAGTAAGAGATAAAGAAATACAC NGG 10 8 17 8 24 0 100 5 10
    240613184652_w63g CTTGCCTACATATGGAGCAGTGACTGCTACTG(+AGGACA)GATCCCTAAGCAATAGATTCATTTGGAGGCA NGG 10 8 17 8 24 0 100 5 10