Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184625_dwme TTCTCCTACTTCTTACCAACCCAGAAGATGGG(+AGGACA)GTACCATTTCAGTCCTGAGTCACCTGAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613184624_xtnt TTCCCAAGCTTTTAGAGGAAAATGAGTCATTG(+AGGACA)GTACCCTTGTGCATTAGTTGTGGTTAATTAG NGG 10 8 17 8 24 0 100 5 10
    240613184622_t9jg AATAACTAATTAACCACAACTAATGCACAAGG(+AGGACA)GTACCAATGACTCATTTTCCTCTAAAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613184620_zcrf GGGATTCAGTGACCTTTCCTGGTTCCCACAGG(+AGGACA)GTACCTGAACTCATGTGTATAGATGCACACA NGG 10 8 17 8 24 0 100 5 10
    240613184618_xkb2 GTGTCTGTGTGCATCTATACACATGAGTTCAG(+AGGACA)GTACCCTGTGGGAACCAGGAAAGGTCACTGA NGG 10 8 17 8 24 0 100 5 10
    240613184617_i6ii AAAGCCATCTGGTTGCCTGTGAACCATTTTAG(+AGGACA)GTACCATTATGGACTAGGCTATTTTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240613184616_9jug ATTAACCAGGTAAAATAGCCTAGTCCATAATG(+AGGACA)GTACCTAAAATGGTTCACAGGCAACCAGATG NGG 10 8 17 8 24 0 100 5 10
    240613184614_mxpd TAATGCCATAAATTTTAAATTGGAAGCACATG(+AGGACA)GATCCCATTTTTCTTTCTACTCCCACATTTT NGG 10 8 17 8 24 0 100 5 10
    240613184613_edk4 GTGAAAAAATGTGGGAGTAGAAAGAAAAATGG(+AGGACA)GATCCATGTGCTTCCAATTTAAAATTTATGG NGG 10 8 17 8 24 0 100 5 10
    240613184610_qitb GGCGGGAAACATGGAGGCCCGGCGGTAAGGGG(+AGGACA)GATCCCGGGCGGGCCAATCTACAAATACGCG NGG 10 8 17 8 24 0 100 5 10