Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184607_98xr GGGGACGCGTATTTGTAGATTGGCCCGCCCGG(+AGGACA)GATCCCCCTTACCGCCGGGCCTCCATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613184606_8z3n CCAACAGGGAAAAGTCACCTGACTCCATTTAG(+AGGACA)GTACCCCAAAATTTCCCAACCATGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240613184604_al74 GGTGTATAGAAACATGGTTGGGAAATTTTGGG(+AGGACA)GTACCTAAATGGAGTCAGGTGACTTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240613184602_prdz GTGGCATGCTTAGGGAAACTCTGGAAAGCTCG(+AGGACA)GTACCATGCAAAGTGCCTGGCCCAGAACTAA NGG 10 8 17 8 24 0 100 5 10
    240613184601_bhez GAAACTTAGTTCTGGGCCAGGCACTTTGCATG(+AGGACA)GTACCGAGCTTTCCAGAGTTTCCCTAAGCAT NGG 10 8 17 8 24 0 100 5 10
    240613184600_phue CCGGGATATACTCTCATTCTCTCTTCTTCAAG(+AGGACA)GTACCTGCATGTGTGTGTGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613184559_dqr7 ACACACACACACACACACACACACACATGCAG(+AGGACA)GTACCTTGAAGAAGAGAGAATGAGAGTATAT NGG 10 8 17 8 24 0 100 5 10
    240613184557_54fd AGCACTGAGGTTGTGCTTCATTGTTGAATTTG(+AGGACA)GTACCACATGTGAAGAAATGATCAAATCAAA NGG 10 8 17 8 24 0 100 5 10
    240613184556_7iv7 ATTTATTTGATTTGATCATTTCTTCACATGTG(+AGGACA)GTACCAAATTCAACAATGAAGCACAACCTCA NGG 10 8 17 8 24 0 100 5 10
    240613184555_2wlz ACAACATTTTAGGACTTAAACATTTGATTTCG(+AGGACA)GTACCAGATGAATTGCTAACCTGTTACTGAT NGG 10 8 17 8 24 0 100 5 10