Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613185010_zban CTCGGTTTGCACACTCTGCCTCAAAAGCCGAG(+AGGACA)GTACCATAAAAACATTGGGAAAGACACAGTG NGG 10 8 17 8 24 0 100 5 10
    240613185008_1yck CTACTATTCAATGACAAATCCCCCAGAGCTGG(+AGGACA)GATCCCAGGGGTACGCTACATCCAGCTGGTG NGG 10 8 17 8 24 0 100 5 10
    240613185004_rdq2 TGTTTCACCAGCTGGATGTAGCGTACCCCTGG(+AGGACA)GATCCCAGCTCTGGGGGATTTGTCATTGAAT NGG 10 8 17 8 24 0 100 5 10
    240613185003_lny1 TGGTACATACATATGCCCTTAGCACTCAGGAG(+AGGACA)GATCCCAGCAAGTCTGAGGTCCACCAGCACT NGG 10 8 17 8 24 0 100 5 10
    240613185001_g09y ACTGTAGTGCTGGTGGACCTCAGACTTGCTGG(+AGGACA)GATCCTCCTGAGTGCTAAGGGCATATGTATG NGG 10 8 17 8 24 0 100 5 10
    240613184959_l9xa GAAAGGTACAGCACGTAAGAACCCTTGATATG(+AGGACA)GTACCTTGCTTGGGCTTCTGGCATGCCTTAC NGG 10 8 17 8 24 0 100 5 10
    240613184958_alpq CCCTAGTAAGGCATGCCAGAAGCCCAAGCAAG(+AGGACA)GTACCATATCAAGGGTTCTTACGTGCTGTAC NGG 10 8 17 8 24 0 100 5 10
    240613184956_lats GGGACATCATATGGCTATAAATCATCATCAGG(+AGGACA)GATCCAGAAAACAGTCAGCGCTGATCACTCG NGG 10 8 17 8 24 0 100 5 10
    240613184954_z16v ACGGCCGAGTGATCAGCGCTGACTGTTTTCTG(+AGGACA)GATCCCTGATGATGATTTATAGCCATATGAT NGG 10 8 17 8 24 0 100 5 10
    240613184951_nidm CTTCAGGCAAGGTCAAGAACAACTTTCCCTTG(+AGGACA)GATCCTCTGAAGCCTGGACTCACCTATTCAA NGG 10 8 17 8 24 0 100 5 10