Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240803152433_dnn9 ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCG(-ATG)GGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240803152411_ekho ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCG(-ATG)GGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240803105157_5tp2 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240803042706_imjf CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240802154708_dzz2 gctctgagttgttatcagtaagggagctgcagtggagtaggcggggagaaggccgcacccttctccggaggggggaggggagtgttgcaatacctttctgggagttctctgctgcctcctggcttctgaggaccgccctgggcctgggagaatc(+ccggatgatcctgacgacggagaccgccgtcgtcgacaagccggcc)ccttccccctcttccctcgtgatctgcaactccagtctttctagagatgggcgggagtcttctgggcaggcttaaaggctaacctggtgtgtgggcgttgtcctgcaggggaattgaacaggtgtaaaattggagggacaagacttcccacagattttcggttttgtcgggaagttttttaataggggcaaataaggaaaatgggaggataggtagtcatctggggttttatgcagcaaaactacaggttattattgcttgtgatccgcctcggagtattttccat NGG 10 8 17 8 75 0 100 5 10
    240802153905_648h gctctgagttgttatcagtaagggagctgcagtggagtaggcggggagaaggccgcacccttctccggaggggggaggggagtgttgcaatacctttctgggagttctctgctgcctcctggcttctgaggaccgccctgggcctgggagaatc(+ccccaactggggtaacctttgagttctctcagttgggg)ccttccccctcttccctcgtgatctgcaactccagtctttctagagatgggcgggagtcttctgggcaggcttaaaggctaacctggtgtgtgggcgttgtcctgcaggggaattgaacaggtgtaaaattggagggacaagacttcccacagattttcggttttgtcgggaagttttttaataggggcaaataaggaaaatgggaggataggtagtcatctggggttttatgcagcaaaactacaggttattattgcttgtgatccgcctcggagtattttccat NGG 10 8 17 8 80 0 100 5 10
    240802153722_ja96 aggggagtgttgcaatacctttctgggagttctctgctgcctcctggcttctgaggaccgccctgggcctgggagaatc(+ccccaactggggta)ccttccccctcttccctcgtgatctgcaactccagtctttctagagatgggcgggagtcttctgggcaggcttaaaggctaacctggtgtgtgggcgttgtcctg NGG 10 8 17 8 24 0 100 5 10
    240802153701_1iie aggggagtgttgcaatacctttctgggagttctctgctgcctcctggcttctgaggaccgccctgggcctgggagaatc(+ccccaactgggg)ccttccccctcttccctcgtgatctgcaactccagtctttctagagatgggcgggagtcttctgggcaggcttaaaggctaacctggtgtgtgggcgttgtcctg NGG 10 8 17 8 24 0 100 5 10
    240802153651_zgec aggggagtgttgcaatacctttctgggagttctctgctgcctcctggcttctgaggaccgccctgggcctgggagaatc(+ccccaact)ccttccccctcttccctcgtgatctgcaactccagtctttctagagatgggcgggagtcttctgggcaggcttaaaggctaacctggtgtgtgggcgttgtcctg NGG 10 8 17 8 24 0 100 5 10
    240802153605_79tf aggggagtgttgcaatacctttctgggagttctctgctgcctcctggcttctgaggaccgccctgggcctgggagaatc(+ccccaact)ccttccccctcttccctcgtgatctgcaactccagtctttctagagatgggcgggagtcttctgggcaggcttaaaggctaacctggtgtgtgggcgttgtcctg NGG 10 8 17 8 24 0 100 5 10