Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613185103_dt01 TTACAGATCCCAAAACTACCAGCCCAGGGATG(+AGGACA)GTACCACCCACAATGAGCCTTCTCTGCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613185101_yy49 ACTCTAGAGAACCCTGACTAATACATAAGTTG(+AGGACA)GTACCAGGGACTGAGGTATTGCTGTGATAGG NGG 10 8 17 8 24 0 100 5 10
    240613185058_qevu TCAGGCCTATCACAGCAATACCTCAGTCCCTG(+AGGACA)GTACCAACTTATGTATTAGTCAGGGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613185057_yc0l GAGCCTTCAGTAGTAATTGGGAAAAGGTAATG(+AGGACA)GATCCATTCATATGCGAAGTAAACATGACAT NGG 10 8 17 8 24 0 100 5 10
    240613185056_bmst TGTCAATGTCATGTTTACTTCGCATATGAATG(+AGGACA)GATCCATTACCTTTTCCCAATTACTACTGAA NGG 10 8 17 8 24 0 100 5 10
    240613185053_h757 TAAGTTGCAGTATGGATCCCAGGATGTTGAAG(+AGGACA)GTACCAGAACTGTGGGTCATCGCCTGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613185052_igou GGGCTTTCCTCAGGCGATGACCCACAGTTCTG(+AGGACA)GTACCTTCAACATCCTGGGATCCATACTGCA NGG 10 8 17 8 24 0 100 5 10
    240613185051_imn6 ACATGTTAAAGTGAAGTCTAAGTTGCAGTATG(+AGGACA)GATCCCAGGATGTTGAAGGTACCAGAACTGT NGG 10 8 17 8 24 0 100 5 10
    240613185049_ao1w GACCCACAGTTCTGGTACCTTCAACATCCTGG(+AGGACA)GATCCATACTGCAACTTAGACTTCACTTTAA NGG 10 8 17 8 24 0 100 5 10
    240613185047_4svr CCAAATAAAAGAACAGGGGAAATTATGGGCAG(+AGGACA)GATCCTAGGCAATAACTAAAGGTATAGGTTG NGG 10 8 17 8 24 0 100 5 10