Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112725_c4gg TAAAGGCAGCTGAGCTGCAGGAGCTCTGACAG(+AGGACA)GATCCGCACGGCATTCCCAGCTGACCGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613112724_2bi4 TGCAGTCTCCGGTCAGCTGGGAATGCCGTGCG(+AGGACA)GATCCTGTCAGAGCTCCTGCAGCTCAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613112722_jafw CATCACAGCAATAGTAACCCTAATGAAGACAG(+AGGACA)GATCCCAGCACCCATGCTGGGTAGCTTACAA NGG 10 8 17 8 24 0 100 5 10
    240613112720_vgz2 TGCTGTTGTAAGCTACCCAGCATGGGTGCTGG(+AGGACA)GATCCTGTCTTCATTAGGGTTACTATTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613112719_l6su TAAGAGAGTTTATTCGGGTTCTGCACTCAGAG(+AGGACA)GTACCTACAACAGACTACAGACAAAATCAAT NGG 10 8 17 8 24 0 100 5 10
    240613112718_eps3 TCCTAATTGATTTTGTCTGTAGTCTGTTGTAG(+AGGACA)GTACCTCTGAGTGCAGAACCCGAATAAACTC NGG 10 8 17 8 24 0 100 5 10
    240613112717_wmxm GACTTTGTACTCTGGCTGTATCCCATCAGAAG(+AGGACA)GTACCATTGTAAGACTCTGGTGAGCCATAGC NGG 10 8 17 8 24 0 100 5 10
    240613112716_2pk8 GGTAAGCTATGGCTCACCAGAGTCTTACAATG(+AGGACA)GTACCTTCTGATGGGATACAGCCAGAGTACA NGG 10 8 17 8 24 0 100 5 10
    240613112714_5js4 CTCACCCCTCCCTTGGCATCCCTATAACTAGG(+AGGACA)GTACCAAGCATGAAGTTCCAGCATGGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240613112713_ieax GAATAACTCCCCATGCTGGAACTTCATGCTTG(+AGGACA)GTACCCTAGTTATAGGGATGCCAAGGGAGGG NGG 10 8 17 8 24 0 100 5 10