Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112748_yfdt CCTCATTATCTACCAAACACATAAGCCAGTGG(+AGGACA)GATCCCACAAGCGTCCCTTGCCTTGTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240613112747_i4kf ATATGTGGGGACAAGGCAAGGGACGCTTGTGG(+AGGACA)GATCCCACTGGCTTATGTGTTTGGTAGATAA NGG 10 8 17 8 24 0 100 5 10
    240613112745_0bvm TCCCATTTGTGTTACTGCGACTGAGAAGGCAG(+AGGACA)GATCCAGGCACCACCTTGAGAGGGAACTGAG NGG 10 8 17 8 24 0 100 5 10
    240613112743_x0s5 TGTGGCTCAGTTCCCTCTCAAGGTGGTGCCTG(+AGGACA)GATCCTGCCTTCTCAGTCGCAGTAACACAAA NGG 10 8 17 8 24 0 100 5 10
    240613112742_6gvz ACCACCACAGCAGCCTATCAGCCACTTCTGGG(+AGGACA)GATCCAGTCCAAGAATGAAAGCCCATAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613112740_7dv0 TCTGCAGCCTATGGGCTTTCATTCTTGGACTG(+AGGACA)GATCCCCAGAAGTGGCTGATAGGCTGCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613112739_c8aw CTGCACAGCACCTCTGAAAGCCACTGGTTGAG(+AGGACA)GTACCTACCTGTAGGGAGAGGGTGTCATTTC NGG 10 8 17 8 24 0 100 5 10
    240613112738_21t3 AGCAAGAAATGACACCCTCTCCCTACAGGTAG(+AGGACA)GTACCTCAACCAGTGGCTTTCAGAGGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613112737_6j78 ACAATAAATGTTTACATTTCTACTAATTTCTG(+AGGACA)GATCCACTCACCTGTAAATATGCTGTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240613112735_rstz AACCTGCTGAACAGCATATTTACAGGTGAGTG(+AGGACA)GATCCAGAAATTAGTAGAAATGTAAACATTT NGG 10 8 17 8 24 0 100 5 10