Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112712_43y3 TCAGATTGTCACAGGAATACCCCACTCTGCTG(+AGGACA)GTACCAATTTGTCTTAGTCAGGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240613112711_k4gw GCAGGAATAGAAACCCTGACTAAGACAAATTG(+AGGACA)GTACCAGCAGAGTGGGGTATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240613112709_fbi3 TTCCTGCCTCTTGCAGAGAGGCTCCAAGTTTG(+AGGACA)GTACCCATGCCAGCTAGCTGGCAGCTACCTG NGG 10 8 17 8 24 0 100 5 10
    240613112708_xbcy GGCTTCAGGTAGCTGCCAGCTAGCTGGCATGG(+AGGACA)GTACCAAACTTGGAGCCTCTCTGCAAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240613112706_qe16 ACTGTCTTCACACACACCAGAAGAGGGCATTG(+AGGACA)GATCCCATTACAGATGTTTGTAAGCTACCAT NGG 10 8 17 8 24 0 100 5 10
    240613112705_q989 GTTGCATGGTAGCTTACAAACATCTGTAATGG(+AGGACA)GATCCAATGCCCTCTTCTGGTGTGTGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613112704_07q8 GGCCAAGTCATGAGAAGCAAGCCAGTAAGCAG(+AGGACA)GATCCCTCCATGGCCCCTACATCAGTTCCTA NGG 10 8 17 8 24 0 100 5 10
    240613112702_dxfm GGAGGTAGGAACTGATGTAGGGGCCATGGAGG(+AGGACA)GATCCTGCTTACTGGCTTGCTTCTCATGACT NGG 10 8 17 8 24 0 100 5 10
    240613112700_hsta CAAAAGTCAATCTCCAACCGTCCAATGTCTGG(+AGGACA)GATCCAACTTACGATCTTCTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613112659_tedr GCCCTTGGAGGAGCCCAGAAGATCGTAAGTTG(+AGGACA)GATCCCAGACATTGGACGGTTGGAGATTGAC NGG 10 8 17 8 24 0 100 5 10