Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112606_jljw TCCTGGCACACAGTAGGTGGCTGTCCCCCGTG(+AGGACA)GTACCACAGTACAGACGGAAGGCGTTCTTGA NGG 10 8 17 8 24 0 100 5 10
    240613112604_xh3m GAGTGTCAAGAACGCCTTCCGTCTGTACTGTG(+AGGACA)GTACCACGGGGGACAGCCACCTACTGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240613112603_5xn1 ACTGGCATGAGTTTCGGCATCTTTGGCAGCAG(+AGGACA)GTACCTTTACCTGCCAAGCCATCTCTACCTT NGG 10 8 17 8 24 0 100 5 10
    240613112602_jm83 CGAATAAGGTAGAGATGGCTTGGCAGGTAAAG(+AGGACA)GTACCTGCTGCCAAAGATGCCGAAACTCATG NGG 10 8 17 8 24 0 100 5 10
    240613112601_2uqb CCCAGCTTCCTGCTTTGTATGAATTGAGTGAG(+AGGACA)GTACCTCTGTCCCTGGTTTCCCTCGCACAAG NGG 10 8 17 8 24 0 100 5 10
    240613112559_s2g6 CAGTGCTTGTGCGAGGGAAACCAGGGACAGAG(+AGGACA)GTACCTCACTCAATTCATACAAAGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613112558_7uk2 CAAGTGTGTGGAGCGCAGAGAACCCCTGGCAG(+AGGACA)GATCCTCACCACCAAAGGGTCCTGGAGACTG NGG 10 8 17 8 24 0 100 5 10
    240613112556_kiy2 CATGCCAGTCTCCAGGACCCTTTGGTGGTGAG(+AGGACA)GATCCTGCCAGGGGTTCTCTGCGCTCCACAC NGG 10 8 17 8 24 0 100 5 10
    240613112532_901r TACTCTACTCCATACCCAGCAGCCCTAGAAGG(+AGGACA)GATCCTGGCCTGCTTTACAGAGAAATGAATG NGG 10 8 17 8 24 0 100 5 10
    240613112530_3ssq AACTTCATTCATTTCTCTGTAAAGCAGGCCAG(+AGGACA)GATCCCTTCTAGGGCTGCTGGGTATGGAGTA NGG 10 8 17 8 24 0 100 5 10