Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112635_eg2d AGGTGATGTAAGGCCAGCAAGTTGGTTCCTGG(+AGGACA)GATCCCCAGGTCTGGCATAGTTGGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613112632_vobs AATGCCAACTATCCAACTATGCCAGACCTGGG(+AGGACA)GATCCCAGGAACCAACTTGCTGGCCTTACAT NGG 10 8 17 8 24 0 100 5 10
    240613112631_381y GATGAAGGGGATCCTTCCACGCACGCACGAAG(+AGGACA)GTACCCTCTGTCTATCCTGGGACCTGACCCC NGG 10 8 17 8 24 0 100 5 10
    240613112629_lbk1 ACTGTGGGGTCAGGTCCCAGGATAGACAGAGG(+AGGACA)GTACCTTCGTGCGTGCGTGGAAGGATCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613112627_2n0x GTTGAGACTAGTGTTACTTTGTAGATGAAGGG(+AGGACA)GATCCTTCCACGCACGCACGAAGGTACCCTC NGG 10 8 17 8 24 0 100 5 10
    240613112626_rjpo AGACAGAGGGTACCTTCGTGCGTGCGTGGAAG(+AGGACA)GATCCCCTTCATCTACAAAGTAACACTAGTC NGG 10 8 17 8 24 0 100 5 10
    240613112624_e318 CTTCTGTGTTACATAGCCATGGTGGGTTGGCG(+AGGACA)GATCCTTCTCGTGGAGTCATTGCATGCTGAC NGG 10 8 17 8 24 0 100 5 10
    240613112623_l3qk TGGCAGTCAGCATGCAATGACTCCACGAGAAG(+AGGACA)GATCCGCCAACCCACCATGGCTATGTAACAC NGG 10 8 17 8 24 0 100 5 10
    240613112621_8jul ATCCTGCTGGTGATACCTAGGAGCTTCAAGGG(+AGGACA)GTACCTAAGTGCTCCAAGAGGTACGTTACAG NGG 10 8 17 8 24 0 100 5 10
    240613112619_a71r GGACCCTGTAACGTACCTCTTGGAGCACTTAG(+AGGACA)GTACCCCTTGAAGCTCCTAGGTATCACCAGC NGG 10 8 17 8 24 0 100 5 10