Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112651_kvpe ACAGCATAGCAGAAGTTTTTAATAGGTGACTG(+AGGACA)GTACCACAGTAAGAAGGAATCATTTCGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613112650_c5e1 ACGGGCAGGCGAAATGATTCCTTCTTACTGTG(+AGGACA)GTACCAGTCACCTATTAAAAACTTCTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240613112649_dydj TCAGCTGTGCTCCAGATGATACAACGCAGTTG(+AGGACA)GTACCTGCAGCCACTGTGCTCTGAGGCTAGG NGG 10 8 17 8 24 0 100 5 10
    240613112647_9x7a CTCACCCTAGCCTCAGAGCACAGTGGCTGCAG(+AGGACA)GTACCAACTGCGTTGTATCATCTGGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240613112645_ta5t TCTCATTGCTTCAGCCTCTTCTCTGCTGGGTG(+AGGACA)GATCCTGTTTCTGACTGTGGCTGTGTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613112644_66y9 AAAGCAGAAGACACAGCCACAGTCAGAAACAG(+AGGACA)GATCCACCCAGCAGAGAAGAGGCTGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240613112643_kgnz CATGAGCCGGGCTGGAAACTTCGTTGATGTGG(+AGGACA)GATCCAGGCTCCATATGTAGTCCTGGAGGAT NGG 10 8 17 8 24 0 100 5 10
    240613112641_pi77 CTGCTATCCTCCAGGACTACATATGGAGCCTG(+AGGACA)GATCCCACATCAACGAAGTTTCCAGCCCGGC NGG 10 8 17 8 24 0 100 5 10
    240613112639_7lhe CGAGGAGGTCGTGCTAGATGGCCTGTTGGTAG(+AGGACA)GATCCATGCTGGGGCTGGGCCTCTTGTACTC NGG 10 8 17 8 24 0 100 5 10
    240613112638_3nmm GCTGTGAGTACAAGAGGCCCAGCCCCAGCATG(+AGGACA)GATCCTACCAACAGGCCATCTAGCACGACCT NGG 10 8 17 8 24 0 100 5 10