Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112424_la8u AGCCTCCACACGAGTGTTCTAGTACATGTTAG(+AGGACA)GATCCTCTCCTGTACCAGTGTCCAGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240613112423_u16a GTCTGTTACACCTGGACACTGGTACAGGAGAG(+AGGACA)GATCCTAACATGTACTAGAACACTCGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240613112422_xy2y TTCCACTCAAATCAGTTGAGAGCCCAAATCAG(+AGGACA)GATCCACATATCATTCATTGTCATTAGATCT NGG 10 8 17 8 24 0 100 5 10
    240613112421_2b3v TATTGAGATCTAATGACAATGAATGATATGTG(+AGGACA)GATCCTGATTTGGGCTCTCAACTGATTTGAG NGG 10 8 17 8 24 0 100 5 10
    240613112420_lj3r GCTGTTAACAGGAGGTCCAAACCTCTTGGAAG(+AGGACA)GTACCAAGCAGCTAATAAAGGCTGGTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613112418_ftgt GATGAAGAAGACCAGCCTTTATTAGCTGCTTG(+AGGACA)GTACCTTCCAAGAGGTTTGGACCTCCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240613112417_o1g1 AGTCTCCCTAGCGCCACTGTGCTGAGAACAGG(+AGGACA)GATCCTCTGGATGAGGAAGCCCTGGCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613112415_4sb4 CTGAGCTGGGGCCAGGGCTTCCTCATCCAGAG(+AGGACA)GATCCCTGTTCTCAGCACAGTGGCGCTAGGG NGG 10 8 17 8 24 0 100 5 10
    240613112414_bs1k CATCTAACAGCAGCTTGAACCTTGGATCTTAG(+AGGACA)GATCCGCTTGACATAGTCTGCTTTCTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240613112413_71kj AAGTTCTCCGAGAAAGCAGACTATGTCAAGCG(+AGGACA)GATCCTAAGATCCAAGGTTCAAGCTGCTGTT NGG 10 8 17 8 24 0 100 5 10