Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112356_nbto AATGTTTAGAGAACAATTAGGTCAATTTCAGG(+AGGACA)GATCCACTTGGTGTATGTGTGGACCTACAGA NGG 10 8 17 8 24 0 100 5 10
    240613112355_emg7 ACCTATCTGTAGGTCCACACATACACCAAGTG(+AGGACA)GATCCCTGAAATTGACCTAATTGTTCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240613112354_p2kv ATGCCTACTTGATTTCTATTAAAAAGAAAAAG(+AGGACA)GTACCAGGTAATTCTATTTGGGAAACGAATG NGG 10 8 17 8 24 0 100 5 10
    240613112352_o4me TAGTGCATTCGTTTCCCAAATAGAATTACCTG(+AGGACA)GTACCTTTTTCTTTTTAATAGAAATCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240613112350_7c2p GTATTTACGGATAGTTGTAAGCCACTGTGTGG(+AGGACA)GTACCGGGAATTGAACCCAGGTCCTCTGCTA NGG 10 8 17 8 24 0 100 5 10
    240613112348_em4n AGCACTAGCAGAGGACCTGGGTTCAATTCCCG(+AGGACA)GTACCCACACAGTGGCTTACAACTATCCGTA NGG 10 8 17 8 24 0 100 5 10
    240613112347_100t TAATGTCCTACTCCTGGCTTGCAATACATGTG(+AGGACA)GATCCTCTTCAGTTATGAATGGAAAATGCCA NGG 10 8 17 8 24 0 100 5 10
    240613112346_6tej TTTCTTGGCATTTTCCATTCATAACTGAAGAG(+AGGACA)GATCCACATGTATTGCAAGCCAGGAGTAGGA NGG 10 8 17 8 24 0 100 5 10
    240613112344_7m84 ATAACATTAACCACATTCTGTTATATAGACTG(+AGGACA)GATCCTTAAAATACACTAATTTATAAATACT NGG 10 8 17 8 24 0 100 5 10
    240613112343_2vwv TTTTCAGTATTTATAAATTAGTGTATTTTAAG(+AGGACA)GATCCAGTCTATATAACAGAATGTGGTTAAT NGG 10 8 17 8 24 0 100 5 10