Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112252_8j05 ATGTAATAGTGTCCAGCCAAACAATCCAACAG(+AGGACA)GATCCTCCACTCTTCTTTCATGTAAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240613112250_ditl TAACTTACAACTTACATGAAAGAAGAGTGGAG(+AGGACA)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    240612033052_82jv AGTGCCTGGTGAAGAATGTGATGGGATCACTAGCATG(+gtgagcggctggcggctgttcaagaagattagc)TCTGCGGAGAGCGGCCCTGGGACGAGATTGAGAAATCTGCCAGTAATGGGGGATGGACTAGAAACTTCCCAAATGTCTACAACACAGGCCCAGGCCCAACCCCAGCCAGCCAACGCAGCCAGCACCAACCCCCCGCCCCCAGAGACCTCCAACCCTAACAAGCCCAAGAGGCAGACCAACCAACTGCAATACCTGCTCAGAGTGGTGCTCAAGACACTATGGAAACACCAGTTTGCATGGCCTTTCCAGCAGCCTGTGGATGCCGTCAAGCTGAACCTCCCT NGG 20 8 25 8 150 0 100 5 10
    240612032521_k7k6 AGTGCCTGGTGAAGAATGTGATGGGATCACTAGCATG(+gtgagcggctggcggctgttcaagaagattagc)TCTGCGGAGAGCGGCCCTGGGACGAGATTGAGAAATCTGCCAGTAATGGGGGATGGACTAGAAACTTCCCAAATGTCTACAACACAGGCCCAGGCCCAACCCCAGCCAGCCAACGCAGCCAGCACCAACCCCCCGCCCCCAGAGACCTCCAACCCTAACAAGCCCAAGAGGCAGACCAACCAACTGCAATACCTGCTCAGAGTGGTGCTCAAGACACTATGGAAACACCAGTTTGCATGGCCTTTCCAGCAGCCTGTGGATGCCGTCAAGCTGAACCTCCCT NGG 20 8 25 8 150 0 100 5 10
    240612010813_ijj6 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240611180309_n62o CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240611163003_5xh8 TCTTTATTTAGAGTGTGTTTAGACATTTAAAATTTTTAAACATGCTCTTTTTGATACAGTCATAATATCATCATTTATGTATTAGTTATATTGCACAAATACTTTCCAGAAGTTATAGCAATATCCTCTACTGTTTACTTAGTACTTGTTTCTAAAGAAGATTGAATTAATCTTCTAAAAAAGAACCTCAAGTACCTGTGTTTCTTGTCTGTTATTATACTCATAGCAGATTGCCAATGTCCTAGAAAGAC(/t)AAAACGTCCAAACTCCAAAGAAAGAATACAATGTGCTCTTTCCACATGTTGGCCGGCAATAATAGCTGAAGGGTGTAGAAGCATGTGAAGATACAATTTCATTTTTCCCAAAGCTTTGTTGGAGGTATAGAAGAAAGTCTAGACATGATACTGGATTAAGAACTAACAACAAGAAGTTAGTGGATTATAATAGCAGGAAAGAATAATCTTCTGAATACTTTGTTGGGCTTACCTTGTATGTCAAATGTCA NGG 30 8 17 8 30 0 300 5 10
    240611162939_p6ji TCTTTATTTAGAGTGTGTTTAGACATTTAAAATTTTTAAACATGCTCTTTTTGATACAGTCATAATATCATCATTTATGTATTAGTTATATTGCACAAATACTTTCCAGAAGTTATAGCAATATCCTCTACTGTTTACTTAGTACTTGTTTCTAAAGAAGATTGAATTAATCTTCTAAAAAAGAACCTCAAGTACCTGTGTTTCTTGTCTGTTATTATACTCATAGCAGATTGCCAATGTCCTAGAAAGAC(/t)AAAACGTCCAAACTCCAAAGAAAGAATACAATGTGCTCTTTCCACATGTTGGCCGGCAATAATAGCTGAAGGGTGTAGAAGCATGTGAAGATACAATTTCATTTTTCCCAAAGCTTTGTTGGAGGTATAGAAGAAAGTCTAGACATGATACTGGATTAAGAACTAACAACAAGAAGTTAGTGGATTATAATAGCAGGAAAGAATAATCTTCTGAATACTTTGTTGGGCTTACCTTGTATGTCAAATGTCA NGG 20 8 17 8 40 0 200 5 10
    240611161726_7ump CCTTCTATTCAATATTCATTTAAAAGGAACATTTCTGATAGCTTCATTTATGCACATTTTCAACAAACAACAACCCAAAGAATCAGAACTTGCCATATTCAATTTTATGCAGCTCACTAAATGACCAATAAGAGAGACAGAGGGCACTTGTACTTTTTCATGCTTAGATTACTGCAAAATGGAACAATTAACAAATGTATGCTGCTAGATTCAGACTCTTTTCTTCCAATATTTTGCACAGATGCCAAATGCAAAATGCACTTTCAAAATGCATTATATTAAGAAATAATTGGTCTTTATTCTAGAAGTTAATTTAACTGATTATTCTAAACAGGTGGCTTATGGCTCTATAAGAACTCCTATTAGTTACTTTGCTTGAGATTATTTTCTAATTTTCTTCTGGAAAAGTTGGGCAAACTGAATGCTTGAAGTTCACTCCTCATGTCTGGTACA(/g)GTATGAGAAAATATTCTTGTTGGCTACTTGACAGGGTAAATAAAATAAGGAACTACTGGGAAAAGAGAATATATTGCTCCCTGAAGGTGCAAGTGTTTCTTCAAGCTGAAAATTACACATTTATGAGAAAAGCCAAGTGCTTCCCAATGGAGAATTGGGTATCTCAAATCAGCTGAAGTTAAATGAAATACATATATTGAATAGATAACTAAATTCCACAATTGGAACTTAAGGAAATGCATTCTCCTCTGTCAAAACGTTTCACATAGGTTC NGG 10 8 17 8 24 0 100 5 10
    240611161629_whwf AGCCATCGTGGCCTTTCACGGTGGCAACTACCGCGAGCTCTATCATATCCTGGAAAACCACAAGTTCACCAAGGAGTCGCACGCCAAGCTGCAGGCGCTGTGGCTTGAAGCACACTACCAGGAGGCTGAGAAGCTGCGTGGAAGACCCCTGGGACCTGTGGACAAGTACCGAGTAAGGAAGAAGTTCCCGCTGCCGCGCACCATTTGGGACGGCGAACAGAAGACACACTGCTTCAAGGAGCGCACGCGGA(/c)ACCTGCTACGCGAGTGGTACCTGCAGGATCCATACCCTAACCCCAGCAAAAAACGTGAGCTCGCCCAGGCAACCGGACTGACCCCTACGCAGGTGGGCAACTGGTTCAAAAACCGCCGACAAAGGGACCGAGCGGCTGCAGCCAAGAACAGGTCGGTACCTAGAGGCCTCCGCGCTTTGAGCGCACCGGGGAGGAGGCGGGTGGAGGCACCTCTGGCGCCCTTACCCAGTCCCTGGCGACTCCAATTCAG NGG 10 8 17 8 24 0 100 5 10