Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044758_rpz9 GATTGAAAAAAATGTTTATTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044757_msu3 ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044755_4i2h ATTGAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044753_bjnh ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044751_61cb ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044750_s9ru ATTGAAAAAAAATGTTTATTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044748_x3rh ATTGAAAAAAAATGGTTGTTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044746_bjv8 ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAACAACCATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044745_81tx TTGAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044743_4kb0 ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10