Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240611161037_3avl GGGTCACTGAAGTTTGCAAAACTTTTTCTGCTCCTGGCTGATTGAGCGAGCCAGAGCCAGCTCATAGCTTGGATTGGAAATCATGTGAAACTCATTTATGCCATTTTGTATTTTCTCTCTTTAACTCCCTATCAGACATTGAGCCGAGTTTGCATCCGAGTCTGGAGTTGGTTTACAAGCATACTGGAAATAAAACTCAATTGGGAGTGAGAATCCAGGTGAACCGAAGTAAACGTGAACTCTTTGTGAAT(/c)CAAATACTGCTGTACGCCTCTTCAAAGCCTACAGGCGCCTGAAAATCTCCCATAAATCCAAGCTGCATTGAAAGGATTTGCTCCAAGCCTTCTACCAGTAGCTTGCATCCACATGCATCAGATTCAGCTGTCTCCTAAATCTGCAAAGTGTTATCTGCCTCACCGTGCACAAACTACTGTCCCAAACTGTATTCAATCCAGATGGGAGGTTTTGTCACTTCCTGACTTCCCTTCCATTGTAAGGATGAAT NGG 30 8 17 8 50 0 200 5 10
    240611160813_1xlp GCCAGTTGGCCAGGTTCCTTCCACAATCATTAACCATTTGTTCCTGCCACCCAATGTCCTTGGTTAAGGTGATCTGACTTAAAGGCAAACAAATGTGGCTGAGTATATTTCAAAGGACACCCTTGTCTTGTTATTTCAAATACTCCCATAATTTGGGGCCCCTTTCATCTTTGTTCCTGCTCTAGGAAGTGCTTGGAAGTTATTTTAATTCAAGTTGGGGGTAAATTCTTATTCTTACCCATTGCTCCCAC(/t)TGAGATGACCTCAAGCAATTCTGAATAAAAAGTTATCACGGCATGAACTGGGCAGCATTAAGGCCTGTGATTTGTTCAACAGTGACATCTTGGCAGAGAAACAGAAAGACATATAATCTACTGAGAATGAAGTGCAAGAAAGTTCACTGGAGGAAGGAAAATAATACTAATCCTTTATTCAATAAGCAGTAAGTCGAATGAGGTGCTTCTTTTTTTAAGGTTTAGTAAGTGAAAAGTAAATTTAAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240611160652_spmx AAAAAAAGTAAACAGTGGGAGTAGAGGAGTCTGTAATGAAACAAATTCCTGGCGTAATCAGTTTGGGAGGGGAAGAGAAGAATTTCAATTACTGTGCTACTCCCATGTCCCTCCCCAAACACACACACTCTCCAGCTGCATTCTGAGGCACTTGGAACTCAGACAACTGAGACGGAAAGTATTTTTAAAATCTAATTTACTTAGTCCCTTTGTTTACTTTGTGCATGTCCCTCATTTTAGACAATGAAAGG(/a)ACATTCTTTATTTGCTCCTGTTTTTTAGAGCTGACACAAGGCAGCAACGTGTGTGCTCTCAGTCACCTCTCTGTGGCTTACAGATACAGGAATGTGTGGTGGTGGTGATGGTGGTGGTGGTGGGTTTTTTCCTGGGATTCTGGAGAATTGTGGCCACTGGAGTCAGACGCCCTTGGGAAGTACTGCCTATGGCCACCGACGCTTAGCTAGAGTTGCAGCCCTCTGTCTTTGTTGACCCTGCTTCAGTATT NGG 10 8 17 8 24 0 100 5 10
    240611160538_tb0c TGTCTTTATTTTAAACAATCTAGTGGTGGTTGAAGAGGCCATCTGGAAAGGTGATGTCAACGGAGGGTAGGGTGGTTGCTGGTGGAGGGGAGTAGCCAGCGCCCCTCCCCTCAGCACACATACCAGCCCCCGCAATTCCAGTTTCATGGTGCCTGAGTTGCTGTCTGGTTGGTTGAACACATTCTCCTCAGCTTATATTTAACTCGTCGCTTGTTCTGGAAAAGTAGTAGTACTGCCTTCTCTGGCCTTTG(/a)TAAACATGATTGCTGGACAAAGATGAGCAGCACGGATCTGCTGCGATGGTCAGAATTCAATTATATTACTCTCTCTGAGGCAAAAAGCACACATTTAATAACTAAATTGGATTCCTTCTACTAAGAGATCCTAAATTCGTGTTTAATAGCCAGCTAAATGTAGAGCTGATTTATATTTATAGGCTGGTTGTAGATTTGGTTGATAGAATATAAAGTTTGATCTGTATGTGGATGGTTTTACTACCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240611160505_mzel TCACTGCCTGCCAAGTTGTACTCCACTGGCACGTGGATGGTGTGACTGCCTTTACACGTGACTGGGAATCTAAACCCCACCCTGCTCAGCGCCATGGCGGGGTGTCTGATGCAGCAGCCGCAGCAACAGCTTCCCAAGTTGTCAGAGAAGAGCCTTAAGCTTCCTTGCTACATGGGACTCCAGGAGTCCCCTGGGAAGTTCAGGCTAGAGCCTGTTTGTTCACCTTCCCCATGATTCCATAAACCATTTAA(/G)TATCCTGAAATAAATCCCTTTCTGCTCTGCTCTGTAACTGAACCCTGACCAACACCGAGACTGGAATCAGGAGAGGTCGTAGGACTGAGTCTTAGGAATCAAAGTCTCAGCTCCCTCGGCTGGGTGGTTTTCAAACTCCTGGGTCCAAATGTATACACATAATTTCAAAGAAATCAATTTCCAGATCCTTACCTCCTTTATACACTCTTGCCTCAAGGCAAGTGTTCAAAAGCACACTTTATAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240611160307_qu0r ATTTCATGATGCCCAAAGGACACATGGGCATCATATTCAGCAAAGACCTTGTGAGAGTTTGATATTACCCTCCCATGGGTTTCAAGCAGTGAGAGGCAAGGAAGCCTCATTTAAAAGTACCTTTGTGATGCCATTAGGTTTCCATTACCTTCATGCAATGCCCGGCGAGCTAAAGCCTCAAATTCTTCAAAACTGTGTAGCAGGTAACAGTGCTCCTCCTTTGGGGTGGAAGCCATGTCATTCAGCTCTCG(/C)AATGTTCCCTTGCCATATGCCAAAAGTGAAGATCTCCACTCCTGAATCTCGCAGTGACGCTGCAATTGGTCTAGGGTCTCCCCCATTGGAATATCCATCAGTGATGAGAAATACAACTTTTGTTGAGTTTTCTCTAGCATGAAGAAGAATTTGCTGTAATGAAATGGGGAAAGTTAATATGAGTGTGTACTGTCTTGCCTACTGTGTGCTTCTCTTCTTACGTAAGGCAGTACTTGCAAAGATCATTAGT NGG 20 8 17 8 40 0 100 5 10
    240611155957_tu92 TCACTGCCTGCCAAGTTGTACTCCACTGGCACGTGGATGGTGTGACTGCCTTTACACGTGACTGGGAATCTAAACCCCACCCTGCTCAGCGCCATGGCGGGGTGTCTGATGCAGCAGCCGCAGCAACAGCTTCCCAAGTTGTCAGAGAAGAGCCTTAAGCTTCCTTGCTACATGGGACTCCAGGAGTCCCCTGGGAAGTTCAGGCTAGAGCCTGTTTGTTCACCTTCCCCATGATTCCATAAACCATTTAA(/g)TATCCTGAAATAAATCCCTTTCTGCTCTGCTCTGTAACTGAACCCTGACCAACACCGAGACTGGAATCAGGAGAGGTCGTAGGACTGAGTCTTAGGAATCAAAGTCTCAGCTCCCTCGGCTGGGTGGTTTTCAAACTCCTGGGTCCAAATGTATACACATAATTTCAAAGAAATCAATTTCCAGATCCTTACCTCCTTTATACACTCTTGCCTCAAGGCAAGTGTTCAAAAGCACACTTTATAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240611155902_wof5 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240611152006_oc6c CAAAAAACTAAAAAACTAGACATTGGTTTAATGGAAACATGAGAGGCAAAGGGATTGTCTCTACAGTTCTACTGTATGAAATAAATAATAGCTTCTTTTGTTTGTTTGTGTTGTTTTTGTTTTTTTGAGACAGAGTCTCACTCTGTTGCCCAGGCTGGAGAGCAATGGCATGATCTTGGCTCACTGGAACCTCTGCCTCCTGGGTTCAAGCAGTTCTCCTGCCTCAGCCTCCAAGTAGCTAGGATTACAGGTGCTCTCCACCACACCTGGCTAATTTTTTGTATTTTCAGTAGAGACAAGGTTTTGCCATGTTGGCCAGGCTGGTCTGAAACT(/C)CCTGAGCCCTAGTGATCCATCCACCTTGGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACTGCACCCGGCCAATAGTTTGGTTTATTAGATGCATCAATAATCAGGGCATTTTGTAGAGGAACCTACTTATCTTTTAATGGAGATAGCATGCAATGGCTACTTCATCTAATTCATTAAAATACTTTTTCTTCCTACATTTATTTATTTATGTATTTATTTTATTTTATTTTTATTTTGAGATGGAGTTTCACTCTTGTTGCCCAGGCTGGAGTGCATTGGTGTGATCTCAGCTCACCACGACCTCTGCCTCCCTGGTTCAAGTGATTCTCCTGCCTCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240611102214_9nq6 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10