Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044741_d63e TTGAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044740_mytd ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044738_lrbf GATTGAAAAAAATGTTTATTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044736_4cvk ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044735_iu0n GATTGAAAAAAATGTTTATTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044733_b7y4 ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044732_7352 TTGAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAAAACCTCAGTA NGG 10 8 17 8 24 0 100 5 10
    240607044729_i8qy AAAGATACTGAGGTTTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044728_w40s TTGAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAAAACCTCAGTA NGG 10 8 17 8 24 0 100 5 10
    240607044726_dm59 AAAGATACTGAGGTTTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10