Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044638_odgd ACTCTAGAGAACCCTGACTAATACATAAGTTG(+ATATAT)GTACCAGGGACTGAGGTATTGCTGTGATAGG NGG 10 8 17 8 24 0 100 5 10
    240607044636_ontw TCAGGCCTATCACAGCAATACCTCAGTCCCTG(+ATATAT)GTACCAACTTATGTATTAGTCAGGGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240607044635_03zu GAGCCTTCAGTAGTAATTGGGAAAAGGTAATG(+ATATAT)GATCCATTCATATGCGAAGTAAACATGACAT NGG 10 8 17 8 24 0 100 5 10
    240607044634_7one TGTCAATGTCATGTTTACTTCGCATATGAATG(+ATATAT)GATCCATTACCTTTTCCCAATTACTACTGAA NGG 10 8 17 8 24 0 100 5 10
    240607044633_qjmi TAAGTTGCAGTATGGATCCCAGGATGTTGAAG(+ATATAT)GTACCAGAACTGTGGGTCATCGCCTGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607044631_c4qj GGGCTTTCCTCAGGCGATGACCCACAGTTCTG(+ATATAT)GTACCTTCAACATCCTGGGATCCATACTGCA NGG 10 8 17 8 24 0 100 5 10
    240607044630_4pd0 ACATGTTAAAGTGAAGTCTAAGTTGCAGTATG(+ATATAT)GATCCCAGGATGTTGAAGGTACCAGAACTGT NGG 10 8 17 8 24 0 100 5 10
    240607044628_p2ae GACCCACAGTTCTGGTACCTTCAACATCCTGG(+ATATAT)GATCCATACTGCAACTTAGACTTCACTTTAA NGG 10 8 17 8 24 0 100 5 10
    240607044627_nonf CCAAATAAAAGAACAGGGGAAATTATGGGCAG(+ATATAT)GATCCTAGGCAATAACTAAAGGTATAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240607044625_pwh7 TTAGACAACCTATACCTTTAGTTATTGCCTAG(+ATATAT)GATCCTGCCCATAATTTCCCCTGTTCTTTTA NGG 10 8 17 8 24 0 100 5 10