Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044551_ywkj CTACTATTCAATGACAAATCCCCCAGAGCTGG(+ATATAT)GATCCCAGGGGTACGCTACATCCAGCTGGTG NGG 10 8 17 8 24 0 100 5 10
    240607044548_2z3m TGTTTCACCAGCTGGATGTAGCGTACCCCTGG(+ATATAT)GATCCCAGCTCTGGGGGATTTGTCATTGAAT NGG 10 8 17 8 24 0 100 5 10
    240607044547_gh38 TGGTACATACATATGCCCTTAGCACTCAGGAG(+ATATAT)GATCCCAGCAAGTCTGAGGTCCACCAGCACT NGG 10 8 17 8 24 0 100 5 10
    240607044545_xtxa ACTGTAGTGCTGGTGGACCTCAGACTTGCTGG(+ATATAT)GATCCTCCTGAGTGCTAAGGGCATATGTATG NGG 10 8 17 8 24 0 100 5 10
    240607044543_6jpe GAAAGGTACAGCACGTAAGAACCCTTGATATG(+ATATAT)GTACCTTGCTTGGGCTTCTGGCATGCCTTAC NGG 10 8 17 8 24 0 100 5 10
    240607044542_zs93 CCCTAGTAAGGCATGCCAGAAGCCCAAGCAAG(+ATATAT)GTACCATATCAAGGGTTCTTACGTGCTGTAC NGG 10 8 17 8 24 0 100 5 10
    240607044541_7dig GGGACATCATATGGCTATAAATCATCATCAGG(+ATATAT)GATCCAGAAAACAGTCAGCGCTGATCACTCG NGG 10 8 17 8 24 0 100 5 10
    240607044539_p1zn ACGGCCGAGTGATCAGCGCTGACTGTTTTCTG(+ATATAT)GATCCCTGATGATGATTTATAGCCATATGAT NGG 10 8 17 8 24 0 100 5 10
    240607044537_gemn CTTCAGGCAAGGTCAAGAACAACTTTCCCTTG(+ATATAT)GATCCTCTGAAGCCTGGACTCACCTATTCAA NGG 10 8 17 8 24 0 100 5 10
    240607044536_6ywc TTGGATTGAATAGGTGAGTCCAGGCTTCAGAG(+ATATAT)GATCCAAGGGAAAGTTGTTCTTGACCTTGCC NGG 10 8 17 8 24 0 100 5 10