Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044653_pduf GATTGAAAAAAATGTTTATCTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCATTATC NGG 10 8 17 8 24 0 100 5 10
    240607044651_ppbb ACAAAGATAATGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAGATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044650_auh1 ATTGAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044649_aj9b ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044647_oh5d TGATCTTGTAATAGTTCTATCTTTAGATCTTG(+ATATAT)GATCCAACTTGCTGCATCTTTCTGTCTGTAG NGG 10 8 17 8 24 0 100 5 10
    240607044646_b6bt AGCTACTACAGACAGAAAGATGCAGCAAGTTG(+ATATAT)GATCCAAGATCTAAAGATAGAACTATTACAA NGG 10 8 17 8 24 0 100 5 10
    240607044644_bwf8 CTGCAGAGACTTGATGTGCCTGGTGGGAGGGG(+ATATAT)GTACCACCCTCTCAGAGAAAAAGGGGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240607044643_djij ATAACTCCCTCCCCTTTTTCTCTGAGAGGGTG(+ATATAT)GTACCCCCTCCCACCAGGCACATCAAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240607044641_z11k GTGATCAAGGCAGAGAAGGCTCATTGTGGGTG(+ATATAT)GTACCATCCCTGGGCTGGTAGTTTTGGGATC NGG 10 8 17 8 24 0 100 5 10
    240607044639_8um7 TTACAGATCCCAAAACTACCAGCCCAGGGATG(+ATATAT)GTACCACCCACAATGAGCCTTCTCTGCCTTG NGG 10 8 17 8 24 0 100 5 10