Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044708_pai7 ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044706_v3bx GATTGAAAAAAATGTTTATTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044704_7rw9 ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044703_9k81 ATTGAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044701_dupb ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ATATAT)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044659_irxn ATTGAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ATATAT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240607044658_qaef ACAGACATACAATATCTCAAGCAGAGGATGTG(+ATATAT)GTACCTGCTCTCTCCTGTCCTTGGAGAGACC NGG 10 8 17 8 24 0 100 5 10
    240607044657_2h5d TGATGGGTCTCTCCAAGGACAGGAGAGAGCAG(+ATATAT)GTACCACATCCTCTGCTTGAGATATTGTATG NGG 10 8 17 8 24 0 100 5 10
    240607044656_pwnp ACAGACATACAATATCTCAAGCAGAGGATGTG(+ATATAT)GTACCTGCTCTCTCCTGTCCTTGGAGAGACC NGG 10 8 17 8 24 0 100 5 10
    240607044655_pao7 TGATGGGTCTCTCCAAGGACAGGAGAGAGCAG(+ATATAT)GTACCACATCCTCTGCTTGAGATATTGTATG NGG 10 8 17 8 24 0 100 5 10