Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044608_v8t7 CATAAGGACCCTTCATGAGGACCCTGCATAAG(+ATATAT)GTACCCAGGAGGATCATCAGGGCTAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240607044606_fybn GGGCCACATTTTAGCCCTGATGATCCTCCTGG(+ATATAT)GTACCTTATGCAGGGTCCTCATGAAGGGTCC NGG 10 8 17 8 24 0 100 5 10
    240607044604_u3pc AAGCAAAAGTATGTCGCCTTTTTCTGAACATG(+ATATAT)GTACCATAGGAATGCTGGTGGCTCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240607044603_i6t5 CACCTCTACACAGAGCCACCAGCATTCCTATG(+ATATAT)GTACCATGTTCAGAAAAAGGCGACATACTTT NGG 10 8 17 8 24 0 100 5 10
    240607044601_w89q TCAAAGCAGGAGAAACTGAAAAGTCACAGCTG(+ATATAT)GTACCAAAGAGCAGGCCAGACTTCACAGCGG NGG 10 8 17 8 24 0 100 5 10
    240607044600_ugoi GAGACCCGCTGTGAAGTCTGGCCTGCTCTTTG(+ATATAT)GTACCAGCTGTGACTTTTCAGTTTCTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240607044557_qcfg TTGGACCTATAGACTGTATTTACGATCCCATG(+ATATAT)GATCCTATCTTACACATAGTAGATATGAAAT NGG 10 8 17 8 24 0 100 5 10
    240607044556_ly3o TAGATATTTCATATCTACTATGTGTAAGATAG(+ATATAT)GATCCATGGGATCGTAAATACAGTCTATAGG NGG 10 8 17 8 24 0 100 5 10
    240607044555_57ga TGCACCACTGTGTCTTTCCCAATGTTTTTATG(+ATATAT)GTACCTCGGCTTTTGAGGCAGAGTGTGCAAA NGG 10 8 17 8 24 0 100 5 10
    240607044553_rk74 CTCGGTTTGCACACTCTGCCTCAAAAGCCGAG(+ATATAT)GTACCATAAAAACATTGGGAAAGACACAGTG NGG 10 8 17 8 24 0 100 5 10