Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044623_8287 CTGAAGAATGTCACCACCTAGGACATGCCTTG(+ATATAT)GTACCTGAAGTTTTCATAAAGGTTTCCATAA NGG 10 8 17 8 24 0 100 5 10
    240607044622_xysi TTCATTTATGGAAACCTTTATGAAAACTTCAG(+ATATAT)GTACCAAGGCATGTCCTAGGTGGTGACATTC NGG 10 8 17 8 24 0 100 5 10
    240607044620_lku8 GGTGTCAGCATTTGGAAGCTGATTATAGGATG(+ATATAT)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607044619_iqlz AGATGGACCATCTAGTGATTGCCATATGCAGG(+ATATAT)GATCCATCCTATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240607044618_19ua TACTGTGATTTTCATAACCCGTTGCCTTTTTG(+ATATAT)GTACCAGAGCTACGTGGTTTGAATCCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240607044616_czm5 CATGTAGCCAGGATTCAAACCACGTAGCTCTG(+ATATAT)GTACCAAAAAGGCAACGGGTTATGAAAATCA NGG 10 8 17 8 24 0 100 5 10
    240607044614_yoqc GGAGTCAAGGACTATAGTCAGGAGACCCCCAG(+ATATAT)GTACCTGGTTCAGAGCAGTCCGGGATGATAG NGG 10 8 17 8 24 0 100 5 10
    240607044612_glv3 TCGTCCTATCATCCCGGACTGCTCTGAACCAG(+ATATAT)GTACCTGGGGGTCTCCTGACTATAGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240607044611_lo0i GTGTAATTGACCTGAAACATATACACGAAAAG(+ATATAT)GTACCCATTTCTTCTAATGCCTTTAAAAATA NGG 10 8 17 8 24 0 100 5 10
    240607044609_4jdx TGGAATATTTTTAAAGGCATTAGAAGAAATGG(+ATATAT)GTACCTTTTCGTGTATATGTTTCAGGTCAAT NGG 10 8 17 8 24 0 100 5 10