Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044448_vwi9 ATCTCAGCCTTGGAATGCATGCACAGACAGGG(+ATATAT)GATCCTGAGAGCAAGCTGGCTAGTGAGACTC NGG 10 8 17 8 24 0 100 5 10
    240607044447_ysbf ACTGTGAGTCTCACTAGCCAGCTTGCTCTCAG(+ATATAT)GATCCCCTGTCTGTGCATGCATTCCAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240607044446_okpv GACTAACTATAAAATGAACAATCTACATATTG(+ATATAT)GATCCATTCATTCCAAGATTAAAATTATCAC NGG 10 8 17 8 24 0 100 5 10
    240607044445_6lsx GTTATGTGATAATTTTAATCTTGGAATGAATG(+ATATAT)GATCCAATATGTAGATTGTTCATTTTATAGT NGG 10 8 17 8 24 0 100 5 10
    240607044443_4raw ACTTGCATGAGTTGATTCTCTCTTACCGCGTG(+ATATAT)GATCCCTGGGATTGAATTCAGGCCACCAGGC NGG 10 8 17 8 24 0 100 5 10
    240607044441_4goc CACGAGCCTGGTGGCCTGAATTCAATCCCAGG(+ATATAT)GATCCACGCGGTAAGAGAGAATCAACTCATG NGG 10 8 17 8 24 0 100 5 10
    240607044440_vu1h ACCTCCAAGAAGCAGGCTTGATGGTCAGAAAG(+ATATAT)GATCCTTGAGGCCAAGGAAGGATCTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240607044439_atcr GACCCCAACAAAGATCCTTCCTTGGCCTCAAG(+ATATAT)GATCCTTTCTGACCATCAAGCCTGCTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240607044437_hhpf AGGGGGGCATGCAATATGGGTGAGGGAGGGCG(+ATATAT)GTACCCTTCTCATCCCAGACCTGAGCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240607044436_jns5 GGGAGGGGGGGCTCAGGTCTGGGATGAGAAGG(+ATATAT)GTACCGCCCTCCCTCACCCATATTGCATGCC NGG 10 8 17 8 24 0 100 5 10