Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044434_2wxt AGGAGAGAAGATGGACACAAGGGCAGATGAGG(+ATATAT)GATCCAGACAAGAAGATAGAGTTCCTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240607044433_xbh5 GAACAATAGCAGGAACTCTATCTTCTTGTCTG(+ATATAT)GATCCCTCATCTGCCCTTGTGTCCATCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607044432_qr06 GCAGGACAATGCACCTTGTCCGGGGCTCATAG(+ATATAT)GATCCAGGAGTGCCCCGAGTTGACAACGAAG NGG 10 8 17 8 24 0 100 5 10
    240607044430_bqim GCCTTCTTCGTTGTCAACTCGGGGCACTCCTG(+ATATAT)GATCCTATGAGCCCCGGACAAGGTGCATTGT NGG 10 8 17 8 24 0 100 5 10
    240607044429_rpdp TTAGGGGAGAGAGTAAGGAGGATACTGATTAG(+ATATAT)GTACCAAGGTTTCATTACCTTAAAGGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240607044427_uf3l CTGGTTTTTGCCTTTAAGGTAATGAAACCTTG(+ATATAT)GTACCTAATCAGTATCCTCCTTACTCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240607044426_81ab ATGGGACCTATTCCAGCCCCTTGAATCTTAAG(+ATATAT)GTACCATATGAGAGCTAAGAGCAGGTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240607044425_xw7i ATGCTGGGAAACCTGCTCTTAGCTCTCATATG(+ATATAT)GTACCTTAAGATTCAAGGGGCTGGAATAGGT NGG 10 8 17 8 24 0 100 5 10
    240607044424_15bx CTTGCTCTCCAGGAGCTCACAGTTCAAGCTGG(+ATATAT)GATCCAGATGAGCAAACATGATCATCACACA NGG 10 8 17 8 24 0 100 5 10
    240607044422_qjyt TATACTGTGTGATGATCATGTTTGCTCATCTG(+ATATAT)GATCCCAGCTTGAACTGTGAGCTCCTGGAGA NGG 10 8 17 8 24 0 100 5 10