Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044421_heve GGTGTCAGAGTTTGGATGCTGATTATGGGGTG(+ATATAT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607044418_r0ld GGATGGACCATGTAGAGACTGCCATATCCAGG(+ATATAT)GATCCACCCCATAATCAGCATCCAAACTCTG NGG 10 8 17 8 24 0 100 5 10
    240607044417_bi6d ACGGACTGCTAAATTGGGTCCTATAGATCGAG(+ATATAT)GTACCATAATTAAGATATTTTAATAAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240607044416_ll0v ATGTTGAGCCTTATTAAAATATCTTAATTATG(+ATATAT)GTACCTCGATCTATAGGACCCAATTTAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607044414_fu6v TATCTCCATGTACTCATGTGAATATAGCCATG(+ATATAT)GATCCTATAGTGAGCTATATCCTTACTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607044413_3f2r AAGGTAAAAAGTAAGGATATAGCTCACTATAG(+ATATAT)GATCCATGGCTATATTCACATGAGTACATGG NGG 10 8 17 8 24 0 100 5 10
    240607044411_zuv8 GCTGATGTGCAGGGGGAAGTAGGATGGCAGGG(+ATATAT)GTACCTAGAGCCCCCTTGATAAATTTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240607044410_lwra AGGACATCTGAAATTTATCAAGGGGGCTCTAG(+ATATAT)GTACCCCTGCCATCCTACTTCCCCCTGCACA NGG 10 8 17 8 24 0 100 5 10
    240607044408_ww31 CAAATTCTTTCCCAGGGTCATGTTGCACCCTG(+ATATAT)GATCCATATGCTACTTTGTTGGTCTCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607044407_tg8k ATTTCTTGCTGAGACCAACAAAGTAGCATATG(+ATATAT)GATCCAGGGTGCAACATGACCCTGGGAAAGA NGG 10 8 17 8 24 0 100 5 10