Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044352_8ev9 AGGTTTTATTGTTCTTAAACAAGGTCAGCCAG(+ATATAT)GTACCAGATTCTAAAAAGAACAATTGCCCCC NGG 10 8 17 8 24 0 100 5 10
    240607044351_6l0d AGGGGGGGGGCAATTGTTCTTTTTAGAATCTG(+ATATAT)GTACCTGGCTGACCTTGTTTAAGAACAATAA NGG 10 8 17 8 24 0 100 5 10
    240607044348_jqz8 GAGTAAGCTCCAGCCCCCAGCACCTTCCCTGG(+ATATAT)GTACCAAGAGTCATCCGAGGGGAGGAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607044347_u16m CTTCCCCAGCTCCTCCCCTCGGATGACTCTTG(+ATATAT)GTACCCAGGGAAGGTGCTGGGGGCTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607044344_p29x CCCATTCGAGCAAATGGCCTGATGGTCCCCAG(+ATATAT)GATCCTTGGTGGAAGTAGGCAGTGTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240607044342_aoxo TGACTTCCTGCACACTGCCTACTTCCACCAAG(+ATATAT)GATCCTGGGGACCATCAGGCCATTTGCTCGA NGG 10 8 17 8 24 0 100 5 10
    240607044340_obe2 TCTGGTGGGGGGCTATGCTGGAGATGAAAGGG(+ATATAT)GATCCCAGCATGAGCCTGGAGGTCCCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240607044339_m4t0 TCCACTGCCAGGGACCTCCAGGCTCATGCTGG(+ATATAT)GATCCCCTTTCATCTCCAGCATAGCCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240607044338_ke6y ACGCTGGTGGAATCCTACGTATTCAAAATTAG(+ATATAT)GATCCTTGTGTAGCACTGAACCTGGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607044337_owno AAATCTCTTTCCCAGGTTCAGTGCTACACAAG(+ATATAT)GATCCTAATTTTGAATACGTAGGATTCCACC NGG 10 8 17 8 24 0 100 5 10