Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044308_o565 GTCCTGCCTACCAACTAGGGCCAAGTCTGCTG(+ATATAT)GATCCTGCCAGTGGGGTCTTAAGTATAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607044306_nlbr CGGGATCTTTATACTTAAGACCCCACTGGCAG(+ATATAT)GATCCAGCAGACTTGGCCCTAGTTGGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240607044305_c82c AAATTGTTCTGTGTATTTGTCACATATTTAAG(+ATATAT)GATCCACGCTTCTTATATTTTCTTTTATTAT NGG 10 8 17 8 24 0 100 5 10
    240607044303_9n0l AACTTATAATAAAAGAAAATATAAGAAGCGTG(+ATATAT)GATCCTTAAATATGTGACAAATACACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240607044302_90i6 GATGTTCATTATTCCTAAATAGCAGCTCAAAG(+ATATAT)GATCCCTTTGCTACACAAGCGCAATGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240607044301_h71p CTTAGGTTTTCATTGCGCTTGTGTAGCAAAGG(+ATATAT)GATCCTTTGAGCTGCTATTTAGGAATAATGA NGG 10 8 17 8 24 0 100 5 10
    240607044300_krrm AATGTGTGTATTTCTTTATCTCTTACTCTATG(+ATATAT)GTACCATGAGATCATTCAGCCGTCCTTGTCA NGG 10 8 17 8 24 0 100 5 10
    240607044259_kby9 ACCTTTGACAAGGACGGCTGAATGATCTCATG(+ATATAT)GTACCATAGAGTAAGAGATAAAGAAATACAC NGG 10 8 17 8 24 0 100 5 10
    240607044257_ye17 CTTGCCTACATATGGAGCAGTGACTGCTACTG(+ATATAT)GATCCCTAAGCAATAGATTCATTTGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607044256_zop4 TGTAATGCCTCCAAATGAATCTATTGCTTAGG(+ATATAT)GATCCAGTAGCAGTCACTGCTCCATATGTAG NGG 10 8 17 8 24 0 100 5 10