Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044239_6xq0 ATGGTGCCCATCAATGGCGTTCCTGCATGGTG(+ATATAT)GTACCAGTCTCAGGCTTTGGGTGCAAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240607044238_uajz TCTCTGTCCTTTGCACCCAAAGCCTGAGACTG(+ATATAT)GTACCACCATGCAGGAACGCCATTGATGGGC NGG 10 8 17 8 24 0 100 5 10
    240607044237_ug36 ATGCACTAACTTTCAAGTCAGTGTGCTGATTG(+ATATAT)GTACCTAAAAAGCTCACCACATTTGCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240607044236_j77s TTCTGCTTCTGCAAATGTGGTGAGCTTTTTAG(+ATATAT)GTACCAATCAGCACACTGACTTGAAAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240607044235_vt5g ATGGAAAACTCAGGTGACTCAGGACTGAAATG(+ATATAT)GTACCCCATCTTCTGGGTTGGTAAGAAGTAG NGG 10 8 17 8 24 0 100 5 10
    240607044233_mc6y TTCTCCTACTTCTTACCAACCCAGAAGATGGG(+ATATAT)GTACCATTTCAGTCCTGAGTCACCTGAGTTT NGG 10 8 17 8 24 0 100 5 10
    240607044232_i3a1 TTCCCAAGCTTTTAGAGGAAAATGAGTCATTG(+ATATAT)GTACCCTTGTGCATTAGTTGTGGTTAATTAG NGG 10 8 17 8 24 0 100 5 10
    240607044230_ebru AATAACTAATTAACCACAACTAATGCACAAGG(+ATATAT)GTACCAATGACTCATTTTCCTCTAAAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240607044228_rnh4 GGGATTCAGTGACCTTTCCTGGTTCCCACAGG(+ATATAT)GTACCTGAACTCATGTGTATAGATGCACACA NGG 10 8 17 8 24 0 100 5 10
    240607044227_48bc GTGTCTGTGTGCATCTATACACATGAGTTCAG(+ATATAT)GTACCCTGTGGGAACCAGGAAAGGTCACTGA NGG 10 8 17 8 24 0 100 5 10