Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044255_vqz2 TTGGATCTAGCACATAATAACAAGGCATAGTG(+ATATAT)GTACCTGCCTGTTCTTGTGCTGAGACGTGTA NGG 10 8 17 8 24 0 100 5 10
    240607044253_p8gf CTGCATACACGTCTCAGCACAAGAACAGGCAG(+ATATAT)GTACCACTATGCCTTGTTATTATGTGCTAGA NGG 10 8 17 8 24 0 100 5 10
    240607044252_92kc CCTGTTCTTGTGCTGAGACGTGTATGCAGTGG(+ATATAT)GATCCTGAGGGCTTTCCTGGACAGTCAATTT NGG 10 8 17 8 24 0 100 5 10
    240607044250_v4go TAGCTAAATTGACTGTCCAGGAAAGCCCTCAG(+ATATAT)GATCCCACTGCATACACGTCTCAGCACAAGA NGG 10 8 17 8 24 0 100 5 10
    240607044249_gnvd ACATGATTACATATATTTGGCAAGTGCACTTG(+ATATAT)GATCCTCCTTACCTGACTGATGAACAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240607044248_gex8 TTATTTTTCTTGTTCATCAGTCAGGTAAGGAG(+ATATAT)GATCCAAGTGCACTTGCCAAATATATGTAAT NGG 10 8 17 8 24 0 100 5 10
    240607044247_iecy TCAGCTGGTATAGAATATGCCTCAAACGCATG(+ATATAT)GATCCCAGGTTTGATCTCAAATGCAGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240607044244_sjk2 GTGCCACACTCTGCATTTGAGATCAAACCTGG(+ATATAT)GATCCATGCGTTTGAGGCATATTCTATACCA NGG 10 8 17 8 24 0 100 5 10
    240607044242_1fg8 GACCATTGTTACTGGCTGAATTAATCTCCAGG(+ATATAT)GTACCTGGAATATTTGAACTGGGGCATGACC NGG 10 8 17 8 24 0 100 5 10
    240607044241_jx0m TCCCAGGTCATGCCCCAGTTCAAATATTCCAG(+ATATAT)GTACCCTGGAGATTAATTCAGCCAGTAACAA NGG 10 8 17 8 24 0 100 5 10