Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044157_fjw9 TGGGAAAGTGTGGGATCACCCCCTGGCCTTTG(+ATATAT)GATCCTCCCCCATATATACTTTCTAACTCTT NGG 10 8 17 8 24 0 100 5 10
    240607044155_ilys CCGCAAAGAGTTAGAAAGTATATATGGGGGAG(+ATATAT)GATCCAAAGGCCAGGGGGTGATCCCACACTT NGG 10 8 17 8 24 0 100 5 10
    240607044154_dqet ATTTAGTGTGTCAAAACGAACCGTACTCAAAG(+ATATAT)GATCCACTAGTGTCCAATTCAAAAATGGCTG NGG 10 8 17 8 24 0 100 5 10
    240607044153_13k2 GGGCACAGCCATTTTTGAATTGGACACTAGTG(+ATATAT)GATCCTTTGAGTACGGTTCGTTTTGACACAC NGG 10 8 17 8 24 0 100 5 10
    240607044152_s5ia TGGATGCTCTCTCCTTAACTGTGGACTTGCAG(+ATATAT)GATCCACATATAGACACTTCAAAGACTTTCC NGG 10 8 17 8 24 0 100 5 10
    240607044150_e379 CACAGGGAAAGTCTTTGAAGTGTCTATATGTG(+ATATAT)GATCCTGCAAGTCCACAGTTAAGGAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240607044149_p7pe CACAGGGAAAGTCTTTGAAGTGTCTATATGTG(+ATATAT)GATCCTGCAAGTCCACAGTTAAGGAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240607044148_em66 TGGATGCTCTCTCCTTAACTGTGGACTTGCAG(+ATATAT)GATCCACATATAGACACTTCAAAGACTTTCC NGG 10 8 17 8 24 0 100 5 10
    240607044146_dseq CACTTAGGTCAGTGTATCAGGCTTGGTGGCAG(+ATATAT)GTACCTGTTCATGCTGAGGCATCGTGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607044145_dzew GTGAGGCTGGCACGATGCCTCAGCATGAACAG(+ATATAT)GTACCTGCCACCAAGCCTGATACACTGACCT NGG 10 8 17 8 24 0 100 5 10