Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044058_l316 AAGCCAACTAGAAAACAACTGGCGTTTATCAG(+ATATAT)GTACCTTAGATTGGTCACTTATACAGCTAAT NGG 10 8 17 8 24 0 100 5 10
    240607044057_53bc ATAAAATTAGCTGTATAAGTGACCAATCTAAG(+ATATAT)GTACCTGATAAACGCCAGTTGTTTTCTAGTT NGG 10 8 17 8 24 0 100 5 10
    240607044055_ik2s ACACAGAGCAACCCTGTCTCTCAATACTAAGG(+ATATAT)GATCCCTAGAGTCAAGGGCTCTGGGTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240607044054_30l3 GGGCAGTTTTACCCAGAGCCCTTGACTCTAGG(+ATATAT)GATCCCTTAGTATTGAGAGACAGGGTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240607044053_2ajo TTCCACACAGAATTATCAGAGATAGGCTATCG(+ATATAT)GATCCTCTTAACTCTGTCTGCTGTTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607044051_nw2p TAGAAAGCTCCAACAGCAGACAGAGTTAAGAG(+ATATAT)GATCCGATAGCCTATCTCTGATAATTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240607044050_m7sn TACAGAGACCTGGGTGACCTTGACCTCATAGG(+ATATAT)GATCCACCTGGTTTTGTTTTTTGTTTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240607044048_nor8 AACCCACAAAAAACAAAAAACAAAACCAGGTG(+ATATAT)GATCCCTATGAGGTCAAGGTCACCCAGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607044046_ag9a AGGGTGTCCCCTCTGAAGAATGCCTGGGTGGG(+ATATAT)GATCCTCAGGGACAACTCCTGCAGGGGTGCA NGG 10 8 17 8 24 0 100 5 10
    240607044045_3xzj CTGCCTGCACCCCTGCAGGAGTTGTCCCTGAG(+ATATAT)GATCCCCACCCAGGCATTCTTCAGAGGGGAC NGG 10 8 17 8 24 0 100 5 10