Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044029_i984 ATCTAGAGGAGGACCTCTAATTTACATACATG(+ATATAT)GATCCATCAACATTCATACATTTATTTACTT NGG 10 8 17 8 24 0 100 5 10
    240607044027_393e GATGGAAGTAAATAAATGTATGAATGTTGATG(+ATATAT)GATCCATGTATGTAAATTAGAGGTCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240607044026_2tsr AGATGGTCACCAACTCTGTAATCTGTAGAGTG(+ATATAT)GATCCACTTGCTCGAGTCACTGGGTCACACG NGG 10 8 17 8 24 0 100 5 10
    240607044025_c1ry CTATTCGTGTGACCCAGTGACTCGAGCAAGTG(+ATATAT)GATCCACTCTACAGATTACAGAGTTGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240607044024_wnur TCTGAAGTAGTAAAGTCCTTTTTCCAGGTTAG(+ATATAT)GTACCAGTTCAGGGTAAGTAACTGGAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240607044023_f2rr GTAGGGTCTCTCCAGTTACTTACCCTGAACTG(+ATATAT)GTACCTAACCTGGAAAAAGGACTTTACTACT NGG 10 8 17 8 24 0 100 5 10
    240607044021_i29q GCTCACAAACATCTATAAATGTAGCTCCATAG(+ATATAT)GATCCAATGCCCCCATCTGCATGCAGACAAA NGG 10 8 17 8 24 0 100 5 10
    240607044020_z8jr GGCATTTTGTCTGCATGCAGATGGGGGCATTG(+ATATAT)GATCCTATGGAGCTACATTTATAGATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240607044017_l6m6 GAACACTTTTTGTTTTATTATTCTAGTCTGGG(+ATATAT)GATCCAGATTCCATAGCCATGGGACTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607044016_h19t CAGGCCCAGCAGTCCCATGGCTATGGAATCTG(+ATATAT)GATCCCCAGACTAGAATAATAAAACAAAAAG NGG 10 8 17 8 24 0 100 5 10