Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044014_qln5 TACAGCATCTGCCATGCTCCCTTCCATAGCCG(+ATATAT)GTACCTTAAGTGAGTTCAATAGTTCCTTTGA NGG 10 8 17 8 24 0 100 5 10
    240607044012_0796 GCCTTTCAAAGGAACTATTGAACTCACTTAAG(+ATATAT)GTACCGGCTATGGAAGGGAGCATGGCAGATG NGG 10 8 17 8 24 0 100 5 10
    240607044011_qcd0 CAAAAATGAAGAGAGAAAACAGGCCTATTGTG(+ATATAT)GTACCTTCTCAACAAGGCTTTCAGTAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240607044009_rpkq AGCAAACTCTTACTGAAAGCCTTGTTGAGAAG(+ATATAT)GTACCACAATAGGCCTGTTTTCTCTCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240607044007_do6e GTAGCTCTTTTCTGTGAGACTCCCAACAGTGG(+ATATAT)GTACCGTGTTTGGCTGATATCCCTGGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240607044006_e6dy GGCAGACCTCCCAGGGATATCAGCCAAACACG(+ATATAT)GTACCCACTGTTGGGAGTCTCACAGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607044005_mpqg GCCACCATACCTAACTTTTACATGGGTGCTAG(+ATATAT)GATCCAAATTCTGGTCCTCCTGCTTCAACTG NGG 10 8 17 8 24 0 100 5 10
    240607044003_pkja TGGCCCAGTTGAAGCAGGAGGACCAGAATTTG(+ATATAT)GATCCTAGCACCCATGTAAAAGTTAGGTATG NGG 10 8 17 8 24 0 100 5 10
    240607044002_qy62 CAAAGGAGATGTAAAAGGGGTAATAGGAGGAG(+ATATAT)GATCCGAGGATTCTGCTGAAGGCCGGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240607044000_dd6f AGTGCAACCCCCGGCCTTCAGCAGAATCCTCG(+ATATAT)GATCCTCCTCCTATTACCCCTTTTACATCTC NGG 10 8 17 8 24 0 100 5 10