Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043958_nr3i GCCCTAGGATTATTTGACTACTGGCCCCAAAG(+ATATAT)GTACCTAAAGGTCAAAATATTTTTCTATCGA NGG 10 8 17 8 24 0 100 5 10
    240607043957_nkk3 CTTTGTCGATAGAAAAATATTTTGACCTTTAG(+ATATAT)GTACCTTTGGGGCCAGTAGTCAAATAATCCT NGG 10 8 17 8 24 0 100 5 10
    240607043955_ydkf ACTGCCCCTGGAATATAAAGCCTTTGATATGG(+ATATAT)GTACCCTGTCTCATGGCCTCTTCTTGGTCGT NGG 10 8 17 8 24 0 100 5 10
    240607043954_pv6e TGGAAACGACCAAGAAGAGGCCATGAGACAGG(+ATATAT)GTACCCATATCAAAGGCTTTATATTCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607043953_850v TAATGAGCCATGGACACTGACCCACCACTGTG(+ATATAT)GATCCACAGACTCAAACAAGGCCCTGATGGC NGG 10 8 17 8 24 0 100 5 10
    240607043951_i16q TCCCTGCCATCAGGGCCTTGTTTGAGTCTGTG(+ATATAT)GATCCACAGTGGTGGGTCAGTGTCCATGGCT NGG 10 8 17 8 24 0 100 5 10
    240607043950_p5it CCAAGTGTGTGAACAGTCTGAGGCCTCACTTG(+ATATAT)GATCCTTACCAAGTCTTTCACAGTTTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240607043949_kc32 CTCAAAAACAAAACTGTGAAAGACTTGGTAAG(+ATATAT)GATCCAAGTGAGGCCTCAGACTGTTCACACA NGG 10 8 17 8 24 0 100 5 10
    240607043948_qaut GGAAATGCAAGCTCAGTCAGCATCTTAAAGAG(+ATATAT)GATCCATGTCCAAATGATGCCTGTCAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240607043947_oryn GCTACCTTGCTGACAGGCATCATTTGGACATG(+ATATAT)GATCCTCTTTAAGATGCTGACTGAGCTTGCA NGG 10 8 17 8 24 0 100 5 10