Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043916_ds7w GTCTTCTGGGAACAGCATTATACCATTGCAGG(+ATATAT)GATCCTCTGCTGAAACTTTTTTAATACTATA NGG 10 8 17 8 24 0 100 5 10
    240607043915_1tol AAAAATATAGTATTAAAAAAGTTTCAGCAGAG(+ATATAT)GATCCCTGCAATGGTATAATGCTGTTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240607043914_06zx ATGTACCCACATCCCCACAAGGGAACTTTAAG(+ATATAT)GATCCACCCCTTAAGCCCCTCCCTCCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240607043912_rrwr TTACAACAGAGGAGGGAGGGGCTTAAGGGGTG(+ATATAT)GATCCTTAAAGTTCCCTTGTGGGGATGTGGG NGG 10 8 17 8 24 0 100 5 10
    240607043911_f7do TCAACCTGGTGGCTCACAACTATCTGTAATGG(+ATATAT)GATCCGATGCCCTTTTCTGATGTGTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240607043910_gf93 GCTGTCTTTAGACACATCAGAAAAGGGCATCG(+ATATAT)GATCCCATTACAGATAGTTGTGAGCCACCAG NGG 10 8 17 8 24 0 100 5 10
    240607043908_i1we GTGGGAGATGTCAGGGCAGGAATTCAAGGCAG(+ATATAT)GATCCTCGATGCAGGAACCAAAGCAGAGACC NGG 10 8 17 8 24 0 100 5 10
    240607043907_gkjk ACCATGGTCTCTGCTTTGGTTCCTGCATCGAG(+ATATAT)GATCCTGCCTTGAATTCCTGCCCTGACATCT NGG 10 8 17 8 24 0 100 5 10
    240607043906_ebc3 TTCAAAAATAGTATAGACAAGGTCAGATGCAG(+ATATAT)GATCCATGTATAGAAAATAAGAAATCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607043905_k788 ATGCCTGGCTGATTTCTTATTTTCTATACATG(+ATATAT)GATCCTGCATCTGACCTTGTCTATACTATTT NGG 10 8 17 8 24 0 100 5 10