Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043836_0oz5 GGTATGCCATATCCCACAGGGGCTATGAGTAG(+ATATAT)GTACCAGATACCAACTCAGTAATTAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240607043834_2uj4 TGCTACAAAATTAATTACTGAGTTGGTATCTG(+ATATAT)GTACCTACTCATAGCCCCTGTGGGATATGGC NGG 10 8 17 8 24 0 100 5 10
    240607043833_q4i1 TGATGGTCAGTGAGGAAGCTGGCCAGCAACTG(+ATATAT)GATCCTCCAGACCCCTCTGCTTCAGGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607043832_vnmj ATAGGGCCTTCCTGAAGCAGAGGGGTCTGGAG(+ATATAT)GATCCAGTTGCTGGCCAGCTTCCTCACTGAC NGG 10 8 17 8 24 0 100 5 10
    240607043830_uee4 ATGTCACCTAGGGAGGACCAAGGATAACCAAG(+ATATAT)GTACCCTTTCTTGGTACTCCCAGGGCCAACC NGG 10 8 17 8 24 0 100 5 10
    240607043829_zsoa TCTAGGGTTGGCCCTGGGAGTACCAAGAAAGG(+ATATAT)GTACCTTGGTTATCCTTGGTCCTCCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240607043827_igi3 CTTAGTCAGGGTCATAAGGTACTTTGGTGGTG(+ATATAT)GATCCTGCTCACTGTTATATAAGTTGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607043826_6ted TGAGACCTGCCAACTTATATAACAGTGAGCAG(+ATATAT)GATCCACCACCAAAGTACCTTATGACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240607043823_7ep8 TCCAGAGGTTTTTACTGAGAGGACTGAGGGGG(+ATATAT)GTACCCCAGCTGGAGGGCTGAAGGCCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240607043821_f6ao GCCTTTCCTGGGCCTTCAGCCCTCCAGCTGGG(+ATATAT)GTACCCCCCTCAGTCCTCTCAGTAAAAACCT NGG 10 8 17 8 24 0 100 5 10