Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043819_e5sl CCTTTTAGCTTGGAGGTGGGAGCCATGCCCAG(+ATATAT)GATCCCTTTCCCGGGCTTTGTCCATGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240607043817_tcie AGGGATTTCCCATGGACAAAGCCCGGGAAAGG(+ATATAT)GATCCTGGGCATGGCTCCCACCTCCAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240607043815_u487 TCTTTCCTCCAGCCCTTTTGGTCTCTTTCCTG(+ATATAT)GATCCTTGTCCTCCAGACTGGCCTCTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607043814_w869 ACTTTCCAGCAGAGGCCAGTCTGGAGGACAAG(+ATATAT)GATCCAGGAAAGAGACCAAAAGGGCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240607043812_dlke GCCTTCCTCAGCCCTGACCTACCTGGCCATAG(+ATATAT)GATCCTACAGCCTTCTCTGTACTCCACTATC NGG 10 8 17 8 24 0 100 5 10
    240607043811_nx6k CAACTGATAGTGGAGTACAGAGAAGGCTGTAG(+ATATAT)GATCCTATGGCCAGGTAGGTCAGGGCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240607043809_lp3k AAACTCAGGTCCTGATATTTGTGAAACAGGTG(+ATATAT)GTACCACTGAGCCAGGTCCATATTCCTCATT NGG 10 8 17 8 24 0 100 5 10
    240607043808_dqg5 GGGTCAATGAGGAATATGGACCTGGCTCAGTG(+ATATAT)GTACCACCTGTTTCACAAATATCAGGACCTG NGG 10 8 17 8 24 0 100 5 10
    240607043806_8i8f ATGCAGAAAAGAGGCCATCCCAACATTTCCAG(+ATATAT)GTACCTTGTTCTCCTAACACAGGCAGCCCAC NGG 10 8 17 8 24 0 100 5 10
    240607043805_bsex TGAGGGTGGGCTGCCTGTGTTAGGAGAACAAG(+ATATAT)GTACCTGGAAATGTTGGGATGGCCTCTTTTC NGG 10 8 17 8 24 0 100 5 10