Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043928_ua1d GCCAAGCTGGTCCTAGGAAGAACCCGAGAGAG(+ATATAT)GATCCGGTGCTCCTTGTCCCATCAGATAGAT NGG 10 8 17 8 24 0 100 5 10
    240607043927_jn6y AAAATAAATAATGCATTAATGCTTCTTCATTG(+ATATAT)GTACCCCCCCCTTTTTTTTGAGACAGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240607043925_o4yi TAGTGAGACTCTGTCTCAAAAAAAAGGGGGGG(+ATATAT)GTACCAATGAAGAAGCATTAATGCATTATTT NGG 10 8 17 8 24 0 100 5 10
    240607043923_6t7l GGAATCTAGATTCCCCCAAGAGTTTAAGGAGG(+ATATAT)GATCCAGAAATCCAAATTTTAAGAACTACAG NGG 10 8 17 8 24 0 100 5 10
    240607043922_ijhg CAATCCTGTAGTTCTTAAAATTTGGATTTCTG(+ATATAT)GATCCCTCCTTAAACTCTTGGGGGAATCTAG NGG 10 8 17 8 24 0 100 5 10
    240607043920_o8rm AAGGCTTCCTTCCATTAATAAATATCATGGTG(+ATATAT)GTACCCCCATGACTAAAAATTAGATTTTCAC NGG 10 8 17 8 24 0 100 5 10
    240607043918_12wk ATTCAGTGAAAATCTAATTTTTAGTCATGGGG(+ATATAT)GTACCACCATGATATTTATTAATGGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607043916_ds7w GTCTTCTGGGAACAGCATTATACCATTGCAGG(+ATATAT)GATCCTCTGCTGAAACTTTTTTAATACTATA NGG 10 8 17 8 24 0 100 5 10
    240607043915_1tol AAAAATATAGTATTAAAAAAGTTTCAGCAGAG(+ATATAT)GATCCCTGCAATGGTATAATGCTGTTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240607043914_06zx ATGTACCCACATCCCCACAAGGGAACTTTAAG(+ATATAT)GATCCACCCCTTAAGCCCCTCCCTCCTCTGT NGG 10 8 17 8 24 0 100 5 10