Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043903_mt2x TGCTGGAGAAGTTGCTGAGAGTTCTATATCTG(+ATATAT)GATCCACAGATATAGCAGGAAGAGAAAGACA NGG 10 8 17 8 24 0 100 5 10
    240607043902_5b1e CCCAGTGTCTTTCTCTTCCTGCTATATCTGTG(+ATATAT)GATCCAGATATAGAACTCTCAGCAACTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607043900_zdvd CTAGTCCTCCCAATGGTAGATAGATTGTCCAG(+ATATAT)GTACCCAGGCAGGAAACCAAAAGTCCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607043858_529v AGGGATTGCTGGACTTTTGGTTTCCTGCCTGG(+ATATAT)GTACCTGGACAATCTATCTACCATTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240607043857_72rb GGAGGAGAGCCTGCAGTTCACCGGCAAGAAAG(+ATATAT)GTACCAGTTGGAGAGAAGAAAAGCTGTACCC NGG 10 8 17 8 24 0 100 5 10
    240607043856_fui3 GGTCTGGGTACAGCTTTTCTTCTCTCCAACTG(+ATATAT)GTACCTTTCTTGCCGGTGAACTGCAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240607043855_zai9 TCCAAAGGTCTGCCCACCTCCTCCAGGGCAAG(+ATATAT)GATCCAATGTCACATCTAAGCATCTGTCCTA NGG 10 8 17 8 24 0 100 5 10
    240607043854_zlla ACCTTTAGGACAGATGCTTAGATGTGACATTG(+ATATAT)GATCCTTGCCCTGGAGGAGGTGGGCAGACCT NGG 10 8 17 8 24 0 100 5 10
    240607043852_mosl GCTGGCCCATTTCATTTTGTCAGGGAGCTTAG(+ATATAT)GTACCTGTGTCCTTGTTTTCCTAAAGCTGAT NGG 10 8 17 8 24 0 100 5 10
    240607043851_gc5u GTGGGATCAGCTTTAGGAAAACAAGGACACAG(+ATATAT)GTACCTAAGCTCCCTGACAAAATGAAATGGG NGG 10 8 17 8 24 0 100 5 10