Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043946_0m2u AAAATGTGGAGAAGTGACAGGAATCAGAAATG(+ATATAT)GATCCACATGAGCCGTCTGATTCTTTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240607043944_5vki ATTCAGAGGCAAAGAATCAGACGGCTCATGTG(+ATATAT)GATCCATTTCTGATTCCTGTCACTTCTCCAC NGG 10 8 17 8 24 0 100 5 10
    240607043943_dr26 CCAGTGCTCTGAACAATATGTCAAGTGCTTGG(+ATATAT)GATCCGCTTTTAAGATGAATTTCTTGAGCAT NGG 10 8 17 8 24 0 100 5 10
    240607043942_ap0n TTAATATGCTCAAGAAATTCATCTTAAAAGCG(+ATATAT)GATCCCAAGCACTTGACATATTGTTCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240607043940_kyxc AGTTTGACCCCAACACAAAGGGGAAAGACAGG(+ATATAT)GTACCTTTCTTCATGAGTTGTCTCCCTACCC NGG 10 8 17 8 24 0 100 5 10
    240607043939_1l5u GGGGGGGGTAGGGAGACAACTCATGAAGAAAG(+ATATAT)GTACCCTGTCTTTCCCCTTTGTGTTGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240607043937_4gfv TGGAGAGGCCAGGACCCCACCTGCTGCATGGG(+ATATAT)GATCCACTAGCCATGGAAGGATACAATAGGA NGG 10 8 17 8 24 0 100 5 10
    240607043936_tfju AGGCTTCCTATTGTATCCTTCCATGGCTAGTG(+ATATAT)GATCCCCATGCAGCAGGTGGGGTCCTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240607043935_0xk0 TTCCATTCAGAAGAATGGGACACAGCTGACAG(+ATATAT)GATCCAGTGCTTGCTAGATTTCATCAGTTCT NGG 10 8 17 8 24 0 100 5 10
    240607043934_p8u5 TCTGAAGAACTGATGAAATCTAGCAAGCACTG(+ATATAT)GATCCTGTCAGCTGTGTCCCATTCTTCTGAA NGG 10 8 17 8 24 0 100 5 10