Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044043_orc7 CCCAAATTCTTCCTTCCATGTAGCTTCACCTG(+ATATAT)GATCCCTTGTGTATTTGAGCTTCAGTTACAG NGG 10 8 17 8 24 0 100 5 10
    240607044041_r5vf AATCTCTGTAACTGAAGCTCAAATACACAAGG(+ATATAT)GATCCAGGTGAAGCTACATGGAAGGAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240607044040_189g AGCCTAACATTAATTAAAACTTTAAGAAAATG(+ATATAT)GTACCTAAGGGCCAAATGAAGTTAGAGTTGA NGG 10 8 17 8 24 0 100 5 10
    240607044038_q8on AAGATTCAACTCTAACTTCATTTGGCCCTTAG(+ATATAT)GTACCATTTTCTTAAAGTTTTAATTAATGTT NGG 10 8 17 8 24 0 100 5 10
    240607044037_en7t AGGTTGATCCTTCCAGCTTTAGGCTGTGCCTG(+ATATAT)GTACCCTGAAGCTAGGCAATTGGAAAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240607044035_0w0l ATGAGACTTCTTTCCAATTGCCTAGCTTCAGG(+ATATAT)GTACCAGGCACAGCCTAAAGCTGGAAGGATC NGG 10 8 17 8 24 0 100 5 10
    240607044034_ceis TGCACCTTTTCTTTTTGTGGCCTACTGTCTTG(+ATATAT)GATCCTCATTTTAGTGCAACAAATGATACAT NGG 10 8 17 8 24 0 100 5 10
    240607044033_tqio ATCAGATGTATCATTTGTTGCACTAAAATGAG(+ATATAT)GATCCAAGACAGTAGGCCACAAAAAGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240607044031_uso0 CAGTGTTCAAACCATACTAACACGACTTGATG(+ATATAT)GTACCCAAAAATATCAACCAACAAAATTTCA NGG 10 8 17 8 24 0 100 5 10
    240607044030_gl97 GAAAATGAAATTTTGTTGGTTGATATTTTTGG(+ATATAT)GTACCATCAAGTCGTGTTAGTATGGTTTGAA NGG 10 8 17 8 24 0 100 5 10