Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607044111_5oje CGCTAACCTCCCCTACTCACCTTGGCAATCTG(+ATATAT)GATCCAATGCTCACTGCCTTCCCTTGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240607044110_zzk8 AACTTAGCCCCAAGGGAAGGCAGTGAGCATTG(+ATATAT)GATCCAGATTGCCAAGGTGAGTAGGGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240607044109_xcfe TGAGAGGGATGTGTGTGTGTGTGTGTGTGTAG(+ATATAT)GTACCTGTAGGGAGAGCAGGGGAGAGAGAAC NGG 10 8 17 8 24 0 100 5 10
    240607044107_b0o9 TTTCTGTTCTCTCTCCCCTGCTCTCCCTACAG(+ATATAT)GTACCTACACACACACACACACACACATCCC NGG 10 8 17 8 24 0 100 5 10
    240607044106_gqfj GGCACAGGCTAACAGAATGGATACTTAAATAG(+ATATAT)GATCCATCATTTTGTTGCATACAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240607044105_q5b5 GAAGTGTGTTTCTTGTATGCAACAAAATGATG(+ATATAT)GATCCTATTTAAGTATCCATTCTGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607044104_sg8t AGAAGATTATACCGTCTCCTCAGCACTTTGTG(+ATATAT)GATCCTTCTCCAAAATTGACCATATACTCAA NGG 10 8 17 8 24 0 100 5 10
    240607044103_b1t6 TATGATTGAGTATATGGTCAATTTTGGAGAAG(+ATATAT)GATCCACAAAGTGCTGAGGAGACGGTATAAT NGG 10 8 17 8 24 0 100 5 10
    240607044101_a0hf GGGTCTGTCCCTGAATCTGTTGCCTGCCTGTG(+ATATAT)GATCCCATTCCCTTAACTTGTCTGGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607044059_4sf7 TCCCACTGAGACCAGACAAGTTAAGGGAATGG(+ATATAT)GATCCACAGGCAGGCAACAGATTCAGGGACA NGG 10 8 17 8 24 0 100 5 10