Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043720_l5cw TTGTGCTTTGTAAAGCAAACTCGAGAAAACTG(+ATATAT)GTACCAGTGTCTGTACCTCAGCTGGGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240607043719_awzc AGATGAAGGACCCAGCTGAGGTACAGACACTG(+ATATAT)GTACCAGTTTTCTCGAGTTTGCTTTACAAAG NGG 10 8 17 8 24 0 100 5 10
    240607043717_rjth ATTCAAAGTTAGAGTGAATCCATGCCAAAAGG(+ATATAT)GATCCCTCACACTGGATAAGAGAAACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240607043716_evcg CTGATCACTGGTTTCTCTTATCCAGTGTGAGG(+ATATAT)GATCCCTTTTGGCATGGATTCACTCTAACTT NGG 10 8 17 8 24 0 100 5 10
    240607043715_2erf AAAGTATAAGCCTGTCAAAGCACGGACTTTAG(+ATATAT)GATCCCCACCAAATATATATACAGATGTACA NGG 10 8 17 8 24 0 100 5 10
    240607043713_kgvk ATATGTGTACATCTGTATATATATTTGGTGGG(+ATATAT)GATCCTAAAGTCCGTGCTTTGACAGGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240607043711_ab5p ACTGTAAAGAAAGCTTCTAGGTCTTCAAAAGG(+ATATAT)GATCCAGAAAAGGGCAAGCCTGAAATGAGGA NGG 10 8 17 8 24 0 100 5 10
    240607043710_lcfd CTAGCTCCTCATTTCAGGCTTGCCCTTTTCTG(+ATATAT)GATCCCTTTTGAAGACCTAGAAGCTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240607043708_uk8f ATGGAGAGAGGGAGACTAATTGGTAAGTGGAG(+ATATAT)GTACCTACCCAAGAAGTTCGGGTGACTCTTT NGG 10 8 17 8 24 0 100 5 10
    240607043707_tfe5 TCTACAAAGAGTCACCCGAACTTCTTGGGTAG(+ATATAT)GTACCTCCACTTACCAATTAGTCTCCCTCTC NGG 10 8 17 8 24 0 100 5 10