Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043617_zri7 TCCTTTTCGCTTAATGAAAGAGGTTGCTTCAG(+ATATAT)GATCCATTGCTGCAGAGAATAATAATTTATT NGG 10 8 17 8 24 0 100 5 10
    240607043616_f4e0 ACAGCAATAAATTATTATTCTCTGCAGCAATG(+ATATAT)GATCCTGAAGCAACCTCTTTCATTAAGCGAA NGG 10 8 17 8 24 0 100 5 10
    240607043615_ks34 CAGTATTCATTCCATGAACATAAGAAATAATG(+ATATAT)GTACCTAAAATTAAAAAACAAAAAACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607043613_hjn0 CAGGTTTTTTGTTTTTTGTTTTTTAATTTTAG(+ATATAT)GTACCATTATTTCTTATGTTCATGGAATGAA NGG 10 8 17 8 24 0 100 5 10
    240607043611_seem TTGCTGCCCATAGAAGCCAGAAGATGGGGTGG(+ATATAT)GATCCCCTGGAACCGGAGTTAGCAGACAGTT NGG 10 8 17 8 24 0 100 5 10
    240607043609_s25o TTCACAACTGTCTGCTAACTCCGGTTCCAGGG(+ATATAT)GATCCCACCCCATCTTCTGGCTTCTATGGGC NGG 10 8 17 8 24 0 100 5 10
    240607043608_t87w CAGTATTCATTCCATGAACATAAGAAATAATG(+ATATAT)GTACCTAAAATTAAAAAACAAAAAACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607043606_po8s CAGGTTTTTTGTTTTTTGTTTTTTAATTTTAG(+ATATAT)GTACCATTATTTCTTATGTTCATGGAATGAA NGG 10 8 17 8 24 0 100 5 10
    240607043605_lqkq TCATTCCTGAATCTCTTTCAAGTTAAAATAAG(+ATATAT)GATCCTAGAATAGCGTCTCAAGCTACAGGTC NGG 10 8 17 8 24 0 100 5 10
    240607043604_r4ff TTTAGGACCTGTAGCTTGAGACGCTATTCTAG(+ATATAT)GATCCTTATTTTAACTTGAAAGAGATTCAGG NGG 10 8 17 8 24 0 100 5 10