Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043518_63dt CCCGACACCAAGGAGAGTGAAATCTGGCTCAG(+ATATAT)GATCCTTGAAGCAGCCACGTTTTCAGTGATT NGG 10 8 17 8 24 0 100 5 10
    240607043517_nit5 GAGGAAATCACTGAAAACGTGGCTGCTTCAAG(+ATATAT)GATCCTGAGCCAGATTTCACTCTCCTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240607043515_dkv1 ATATTTCTAATGCATTGACTTTTCTTCTCCTG(+ATATAT)GTACCAAGTGCTAACTGTGGAATCTAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607043514_roag CAGCGTCCACTAGATTCCACAGTTAGCACTTG(+ATATAT)GTACCAGGAGAAGAAAAGTCAATGCATTAGA NGG 10 8 17 8 24 0 100 5 10
    240607043513_2fsg GTTTTGGATTTGTCCCATTTAAAGAACTAATG(+ATATAT)GATCCAGGGAAAATAAAGTATCCTTGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240607043510_fyo2 TGAGATCAAACAAGGATACTTTATTTTCCCTG(+ATATAT)GATCCATTAGTTCTTTAAATGGGACAAATCC NGG 10 8 17 8 24 0 100 5 10
    240607043508_ez6e TGAAAGCATGTTAAGCACTAATACTAACCATG(+ATATAT)GATCCTTATCCCTTAAGCAATAAAAAGATGT NGG 10 8 17 8 24 0 100 5 10
    240607043507_3ran AATGCACATCTTTTTATTGCTTAAGGGATAAG(+ATATAT)GATCCATGGTTAGTATTAGTGCTTAACATGC NGG 10 8 17 8 24 0 100 5 10
    240607043506_meqm ATCGTCTGGCTATGGCGCCTTGAGGAAGCGTG(+ATATAT)GATCCCTGGGGCTTCTCCATAGCCTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240607043503_yaiu TCTCTCTGTGAAGGCTATGGAGAAGCCCCAGG(+ATATAT)GATCCACGCTTCCTCAAGGCGCCATAGCCAG NGG 10 8 17 8 24 0 100 5 10