Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043413_c4u7 CCTCACCTGCCACCATCTCCTGAACTCTCTGG(+ATATAT)GTACCTGGTATTCCTTATGCATGTGCAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607043411_yqjl GTGATTTTTTGCACATGCATAAGGAATACCAG(+ATATAT)GTACCCAGAGAGTTCAGGAGATGGTGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607043409_n4vx GAAGCAGGCTTTCACACATACACACTCAGGTG(+ATATAT)GATCCTGCACTGACTCTGGAGAAGGCATACA NGG 10 8 17 8 24 0 100 5 10
    240607043408_d45s TATAATGTATGCCTTCTCCAGAGTCAGTGCAG(+ATATAT)GATCCACCTGAGTGTGTATGTGTGAAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607043407_67j2 GTGGCCAACTTTATTTTTACGTGGAACATTAG(+ATATAT)GTACCAAGTTTACAACCATCAAGCAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240607043406_6qnv GAACTATGTTTTGCTTGATGGTTGTAAACTTG(+ATATAT)GTACCTAATGTTCCACGTAAAAATAAAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607043404_h2j0 GGGGTTGTCGCCGCTTTTACGTGAGCGGCGTG(+ATATAT)GATCCACGCAGCGATGCCGTCTACTTTGAGA NGG 10 8 17 8 24 0 100 5 10
    240607043403_49n7 ACCGCTCTCAAAGTAGACGGCATCGCTGCGTG(+ATATAT)GATCCACGCCGCTCACGTAAAAGCGGCGACA NGG 10 8 17 8 24 0 100 5 10
    240607043402_kv9a CCTTTTAGAAATAGGGTCTCCCTCTCTGTGTG(+ATATAT)GTACCACTGGCTAAGGAAACCGTACTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240607043400_q4le AGGCCTCCCAAGTACGGTTTCCTTAGCCAGTG(+ATATAT)GTACCACACAGAGAGGGAGACCCTATTTCTA NGG 10 8 17 8 24 0 100 5 10