Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043329_qsex TACAATCCATTGCTTCCATGAGAGATGGGCAG(+ATATAT)GTACCTATCGGATGTGTGCTCTGCATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240607043327_fsn6 GGAGAGAAACATGCAGAGCACACATCCGATAG(+ATATAT)GTACCTGCCCATCTCTCATGGAAGCAATGGA NGG 10 8 17 8 24 0 100 5 10
    240607043326_7p2w GTTTAGTAGTAATGGCAACAACATTAAGACAG(+ATATAT)GATCCTCATATGAGGACCATGTGCATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240607043325_0kdo AATGTCAACCATGCACATGGTCCTCATATGAG(+ATATAT)GATCCTGTCTTAATGTTGTTGCCATTACTAC NGG 10 8 17 8 24 0 100 5 10
    240607043324_k8sz TGGTAAATAAGGCCTTCCTAGATGTGCACATG(+ATATAT)GTACCAGGAGAATTGTTGCCCTGGGACAAAG NGG 10 8 17 8 24 0 100 5 10
    240607043322_f927 TGGACCTTTGTCCCAGGGCAACAATTCTCCTG(+ATATAT)GTACCATGTGCACATCTAGGAAGGCCTTATT NGG 10 8 17 8 24 0 100 5 10
    240607043320_j5h0 ACTCTCAAGGCATGACCTGAAATTGGAAATGG(+ATATAT)GTACCATCTCTCCCCTCAGTCACTGGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240607043319_b1ze TTGAAGCTTTCCAGTGACTGAGGGGAGAGATG(+ATATAT)GTACCCATTTCCAATTTCAGGTCATGCCTTG NGG 10 8 17 8 24 0 100 5 10
    240607043317_qoba AAACACGGCTTTCCTAAGTAACTGTACCAATG(+ATATAT)GATCCTAAAGTCCCAAATGTAGGCCCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240607043316_lykg TTTGTTTCAAGGGCCTACATTTGGGACTTTAG(+ATATAT)GATCCATTGGTACAGTTACTTAGGAAAGCCG NGG 10 8 17 8 24 0 100 5 10