Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043244_74re TGTTAGCATCGGAAATTGAGGCAAAACCATTG(+ATATAT)GATCCAAGAAGCCAGGCCTGTTTCTTTAACA NGG 10 8 17 8 24 0 100 5 10
    240607043243_qy1e ATAGTTGTTAAAGAAACAGGCCTGGCTTCTTG(+ATATAT)GATCCAATGGTTTTGCCTCAATTTCCGATGC NGG 10 8 17 8 24 0 100 5 10
    240607043241_82h7 TAGGAAGATATTACCAACCCTGCTCAAACAGG(+ATATAT)GTACCAAAGGAACGGTTAAGGTCCCCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240607043239_2dfw GGTTAAGAGAGGGGACCTTAACCGTTCCTTTG(+ATATAT)GTACCCTGTTTGAGCAGGGTTGGTAATATCT NGG 10 8 17 8 24 0 100 5 10
    240607043238_goc4 TGTTCCAAGACACACCCCTTCTAGAGATGCTG(+ATATAT)GATCCAACCCAAACCTGCCCACTCCGGAACC NGG 10 8 17 8 24 0 100 5 10
    240607043237_yo90 TGGTGGGTTCCGGAGTGGGCAGGTTTGGGTTG(+ATATAT)GATCCAGCATCTCTAGAAGGGGTGTGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240607043235_uje9 TATTGGATGGGCACTGGGGGCCAGGGGCCTTG(+ATATAT)GATCCCAAATAAAAGAGAAGTTCTTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240607043233_a1lp GTGGCCCACAGAAGAACTTCTCTTTTATTTGG(+ATATAT)GATCCAAGGCCCCTGGCCCCCAGTGCCCATC NGG 10 8 17 8 24 0 100 5 10
    240607043231_huo3 TATTGGATGGGCACTGGGGGCCAGGGGCCTTG(+ATATAT)GATCCCAAATAAAAGAGAAGTTCTTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240607043230_a923 GTGGCCCACAGAAGAACTTCTCTTTTATTTGG(+ATATAT)GATCCAAGGCCCCTGGCCCCCAGTGCCCATC NGG 10 8 17 8 24 0 100 5 10