Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043157_xzuo CTAAACAAACTTGGAGGTAGTCACTCAGGATG(+ATATAT)GATCCCAATTCACACTTCTCCATAGTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607043155_xnla CAATCATGGAACTATGGAGAAGTGTGAATTGG(+ATATAT)GATCCATCCTGAGTGACTACCTCCAAGTTTG NGG 10 8 17 8 24 0 100 5 10
    240607043154_ztqt GGAGGCCTAGAGAGCTCACAGTATTGAGTGAG(+ATATAT)GTACCCCTTCCACTGGTTTCCATAGCACCCC NGG 10 8 17 8 24 0 100 5 10
    240607043152_njm2 GAAATGGGGTGCTATGGAAACCAGTGGAAGGG(+ATATAT)GTACCTCACTCAATACTGTGAGCTCTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240607043150_oe6h GTCCAGGTCTATATATAGTCAACTCTCTTAGG(+ATATAT)GATCCCCATCTCCCTTCCTCTGGGCCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240607043148_zckx TACAGAGCAAGGCCCAGAGGAAGGGAGATGGG(+ATATAT)GATCCCTAAGAGAGTTGACTATATATAGACC NGG 10 8 17 8 24 0 100 5 10
    240607043147_dndq AAGTTCTTGTTCTCTGAGTCAGAGCTGGGTGG(+ATATAT)GTACCACTGTGCTGGTACTGAAAGGCGTGGG NGG 10 8 17 8 24 0 100 5 10
    240607043145_kgig CCTGCCCCACGCCTTTCAGTACCAGCACAGTG(+ATATAT)GTACCCACCCAGCTCTGACTCAGAGAACAAG NGG 10 8 17 8 24 0 100 5 10
    240607043143_f5f6 TAGAGACTGCCAACAGTGGGAGCTACTCCCTG(+ATATAT)GATCCCTCTTGGGCCGAGTAGTTTTTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240607043142_nynd ATTTGGCTTTAAAAACTACTCGGCCCAAGAGG(+ATATAT)GATCCAGGGAGTAGCTCCCACTGTTGGCAGT NGG 10 8 17 8 24 0 100 5 10