Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043228_rppj ATTGCGGACCATGGATATATTTTATTAACTGG(+ATATAT)GTACCCAAAACACATTTCCCATATTTGGATG NGG 10 8 17 8 24 0 100 5 10
    240607043227_7evj TTCACCATCCAAATATGGGAAATGTGTTTTGG(+ATATAT)GTACCCAGTTAATAAAATATATCCATGGTCC NGG 10 8 17 8 24 0 100 5 10
    240607043225_ynx3 TGGTAGTGTTCAGCGTTGCCGTCAAGTAGAGG(+ATATAT)GTACCTCAAAATATATGGTCCTTGTCCCTCG NGG 10 8 17 8 24 0 100 5 10
    240607043224_02oz GAGAACGAGGGACAAGGACCATATATTTTGAG(+ATATAT)GTACCCTCTACTTGACGGCAACGCTGAACAC NGG 10 8 17 8 24 0 100 5 10
    240607043223_4yaw TGGGAAAGACTCCTCTGAGAAAATTTAAGATG(+ATATAT)GATCCCAACTCTGTGAGTGTGTCTGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607043221_pfjq GAATACCTGCACAGACACACTCACAGAGTTGG(+ATATAT)GATCCATCTTAAATTTTCTCAGAGGAGTCTT NGG 10 8 17 8 24 0 100 5 10
    240607043220_v1br CTCTAGTGGAGGCTTGGTATTAGAAAAGTCTG(+ATATAT)GTACCCCTTAGGCACCTTTCTTGGCATATGA NGG 10 8 17 8 24 0 100 5 10
    240607043218_dba4 TAACCTCATATGCCAAGAAAGGTGCCTAAGGG(+ATATAT)GTACCAGACTTTTCTAATACCAAGCCTCCAC NGG 10 8 17 8 24 0 100 5 10
    240607043217_rt8x GAGCCCTTTGTACTCTATAAACAAAATGCTAG(+ATATAT)GATCCCATCTTAATATTTTTTAAAAGTAATT NGG 10 8 17 8 24 0 100 5 10
    240607043215_jz56 TTTTAAATTACTTTTAAAAAATATTAAGATGG(+ATATAT)GATCCTAGCATTTTGTTTATAGAGTACAAAG NGG 10 8 17 8 24 0 100 5 10